Dear mothur-forum,
we are aware that the removal of singletons is a hot discussed topic. Still, we would like to take that road. 
We have used the split.abund command on our dataset consisting of 3 samples to remove the singletons. However, we have noticed that the command only considers the total sum of the representative sequences in the count_table-file. The result is that we have still singletons in our dataset.
For example:
Representative_Sequence total mock na1 na2
M0300__10262_11047_3333 5 0 1 4
So we would like to remove only the singelton in sample na1 but keep the 4 sequences in sample na2.
Are there any solutions or commands to solve that?
Any tips or hints are welcomed!
Regards,
Beate, Nadja, Götz, Janosch