Sorry if this is a ridiculous question, but I am completely new to bioinformatics & relatively new to molecular biology, coming from a more traditional Ecology background. I have fungal ITS sequences produced from the Sanger method in an experimental design using leaf discs incubated in microcosms & I am now ready to start analyzing the community.
I’ve noticed that there are SOP’s for 454, Illumina, & other sequencing platforms, but I have yet to find any SOP for sequences produced using the Sanger method. Does an SOP exist for this? If not, what is the best approach to take when coming up with a procedure?
Again, sorry if this is an elementary question, but I’m definitely learning “on-the-fly”.