Shhh.flows error: "Segmentation fault (core dumped)"

1

Hi All,
I am new user in mothur and trying to analyse my 454 GS FLX Titanium 16S sequencing data. In interactive mode, I run these commands in order:

sffinfo(sff=PRJNA423040.sff, flow=T)

trim.flows(flow=PRJNA423040.flow, oligos=PRJNA423040.oligos, pdiffs=2, bdiffs=1, processors=2, minflows=225, maxflows=225)

shhh.flows(file=PRJNA423040.flow.files, lookup=/usr/bin/mothur)

after running shhh.flows, clustering flowgrams always give me this error:

PRJNA423040.trim.shhh.dist is blank. Please correct.

and after finalizing procedure, this comes up: “Segmentation fault (core dumped)” and quit from mothur itself.
How can I deal with this kind of problem, I would appreciate any help.
Edit: my laptop has 64 gb ram

Thank you
Seda

2

Could you send your *.sff file and logfile to mothur.bugs@gmail.com