I am a complete novice when it comes to metagenomics but I have been playing with Mothur for a few months to complete some analysis for my thesis. I am about done but my advisor is asking for a different approach that I don’t know how to do.
Is there a way to calculate the Shannon Index and Inv Simpson for several samples combined?
I can only explain what I mean by explaining my project:
I have separate microbial communities from three wipes that were grown in two salt treatments. I extracted DNA from the cultures over six months and we are observing the succession of the communities through who is present and how the diversity changes over time. I have already calculated the diversity for each time point of each wipe of both salt treatments.
My advisor would like me to instead, calculate the Shannon Index and Inv Simpson for combined wipe samples at each time point in both salts. So no longer breaking it down by wipe sample, only time points (so like week 1, week 2, week 3 … ) for each salt treatment.
This is what my table currently looks like (separated by wipe samples)
Instead, I’d like to have just one “combined wipe sample” that would be separated by all five timepoints. (Planned to embed and image here but it won’t let me add more than one).
I hope this makes sense.
Signed - a desperate (and sleepy) grad student.