Hello
I am trying to get the reverse complement of specific three sequences out of my aligned 261 seqs. Those three sequences have been listed in an ACCNOS file, but I am not sure how I can use this file to perform the flipping. My understanding is that “reverse.seqs” flips all the sequences in the file and that “flip” is an option that goes together with the “trim” command. Any suggestion on the language I have to use?
Thanks
Letizia
If you have a list of the accession numbers you can use the get.seqs command to get those sequences and then do reverse.seqs.
Hope this helps,
Pat
Yes it helped, thanks!
However, for some reasons that I do not know, I was not able to “merge” the files back, so I did cut and paste.
Letizia