Regarding "pre.cluster" command

Hi community!!!
As far as the “pre.cluster” command is concerned, what happens with the sequences wih PCR errors? Are they merged with the original sequences or they are removed?

Thanks & Regards,


pre.cluster uses the most abundant sequence as the representative sequence for that cluster. Theoretically PCR errors will be less common so shouldn’t be the rep sequence for a large cluster.

So, are they removed?

probably :woman_shrugging:

they are merged. the number of sequences going into pre.cluster is the same as the number at the end.

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