Hi community!!!
As far as the “pre.cluster” command is concerned, what happens with the sequences wih PCR errors? Are they merged with the original sequences or they are removed?
Thanks & Regards,
DC
pre.cluster uses the most abundant sequence as the representative sequence for that cluster. Theoretically PCR errors will be less common so shouldn’t be the rep sequence for a large cluster.
So, are they removed?
probably 
they are merged. the number of sequences going into pre.cluster is the same as the number at the end.
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