I am posting this to get support about the use of RDP. I am in fact running a mothur pipeline following MiSeq SOP, but instead of using Silva when I get to pcr.seqs, I would like to use RDP database. I retrieve it from the official page in mothur webisite, but mothur gives me an error message saying sequences are not aligned. How should I solve the problem to reach the end of my pipeline?
Thanks in advance!
Note: I am working with Mothur version 1.48 used in a singularity container found on docker, at link Docker and perfectly functioning with other databases.
Hey @pschloss I created a new thread as I was commenting an older one, like from 8 years ago. Hope this can stand better. Thanks!