Questions About shhh.flows

Commands in mothur
1

Hi

I’ve got 2 question about this command

1st:
Is there a way to lauch a single flow file on multiple processors (like 8 processors being used to denoise 1 .flow)

2nd:
I know that shhh.flow is modeling the error when denoising, so the more information there is, the better is the denoising, but I want to know what is the minimum sequences in a flow file to be sure to have enough error modeling and have the best denoising

Clearly, is there a time where the error doesn’t change anymore ?

thanks in advance

2

Is there a way to lauch a single flow file on multiple processors (like 8 processors being used to denoise 1 .flow)

The fact is that it doesn’t really matter. We’ve run a single file on many processors and there’s no improvement. This turns out to be a product of the big compute steps being in the steps that are only run on a single processor.

Clearly, is there a time where the error doesn’t change anymore ?

Sounds like a great experiment that you could run :slight_smile: Sorry, we don’t know.