Dear mothur community,
Since I had a distance matrice too large (315 Gb) to load it in the RAM of my server (126 Gb of RAM), I used the cluster.split command with a classification based on the fasta (taxlevel=3), and the opticlust algorithm. It went smoothly and I succeed to reach the OTU table step.
Now, I would like to know the implication of this alternative method in terms of clustering quality ? And how to manage it properly in the rest of the pipeline and interpretation of the results.
I believe to already read something about it through a previous post in the forum, but I cannot find it back… also I apologize for an eventual duplicated topic.