Dear All,
I tried to run MiSeq_SOP but stuck at cluster.split command. Earlier I have succesfully executed this programme till last step.i thought that some data got corrupted so I freshly downloaded those data but problem persisted the same. Please help me. I am pasting the log file before termination of my programme run. Please suggest me where is the problem.
Thanks
Log file is showing
mothur > dist.seqs(fasta=stability.files/fileList.paired.trim.contigs.good.unique.good.filter.precluster.pick.pick.pick.pick.fasta, cutoff=0.20)
Using 10 processors.
Output File Names:
stability.files/fileList.paired.trim.contigs.good.unique.good.filter.precluster.pick.pick.pick.pick.dist
It took 1 seconds to calculate the distances for 22 sequences.
mothur > cluster(column=stability.files/fileList.paired.trim.contigs.good.unique.good.filter.precluster.pick.pick.pick.pick.dist, count=stability.files/fileList.paired.trim.contigs.good.unique.good.filter.precluster.denovo.uchime.pick.pick.count_table)
********************###########
Reading matrix: |||||||||||||||||||||||||||||||||||||||||||||||||||
changed cutoff to 0.184694
Output File Names:
stability.files/fileList.paired.trim.contigs.good.unique.good.filter.precluster.pick.pick.pick.pick.an.unique_list.list
It took 0 seconds to cluster
mothur > cluster.split(fasta=stability.files/fileList.paired.trim.contigs.good.unique.good.filter.precluster.pick.pick.pick.pick.fasta, count=stability.files/fileList.paired.trim.contigs.good.unique.good.filter.precluster.denovo.uchime.pick.pick.count_table, taxonomy=stability.files/fileList.paired.trim.contigs.good.unique.good.filter.precluster.pick.pds.wang.taxonomy, taxlevel=3, cutoff=0.15)
Using 10 processors.
Using splitmethod fasta.
Splitting the file…
ERROR: M00967_43_000000000-A3JHG_1_1101_10133_8460 is missing from your fastafile. This could happen if your taxonomy file is not unique and your fastafile is, or it could indicate and error.