Hi guys, I have a problem with running cluster.split of mothur. I used “cluster.split(fasta=ok.abund.fasta, count=ok.abund.count_table, taxonomy=ok.taxonomy, taxlevel=4, cutoff=0.03, processors=10)” and give this error:
Selecting sequences for group Gammaproteobacteria_unclassified (12 of 101)
Number of unique sequences: 10484
[WARNING]: ok.abund.count_table does not contain any sequence from the .accnos file.
Selected 0 sequences from ok.abund.count_table.
Calculating distances for group Gammaproteobacteria_unclassified (12 of 101):
[ERROR]: you must have at least 2 sequences to calculate the distances, aborting.
Segmentation fault (core dumped)
Also, I wonder why the warning is about .accnos file. Does cluster.split use a .accnos file?
somebody to help me, please?