Problem cluster.split

Commands in mothur
1

Hi guys, I have a problem with running cluster.split of mothur. I used “cluster.split(fasta=ok.abund.fasta, count=ok.abund.count_table, taxonomy=ok.taxonomy, taxlevel=4, cutoff=0.03, processors=10)” and give this error:

Selecting sequences for group Gammaproteobacteria_unclassified (12 of 101)
Number of unique sequences: 10484

[WARNING]: ok.abund.count_table does not contain any sequence from the .accnos file.
Selected 0 sequences from ok.abund.count_table.

Calculating distances for group Gammaproteobacteria_unclassified (12 of 101):
[ERROR]: you must have at least 2 sequences to calculate the distances, aborting.
Segmentation fault (core dumped)

Also, I wonder why the warning is about .accnos file. Does cluster.split use a .accnos file?

somebody to help me, please?

2

Is this the same dataset as you were struggling with on the other thread? Can you revisit this once you get that thread resolved?

Pat

3

This is my first thread…what do you mean with "the other thread’'?

4

Ack - sorry, I got you confused with someone else.

Can you post the command as you are running it along with which version number and operating system you are using?

Pat

5

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