i’ve got a question because i’m not completely convinced by some papers that i’ve read…
following a experimental design from a natural bacterial community (16s), we have a sample at time 0 without replication due to the difficulty to recover DNA sequences, and samples at time 7 after a specific treatment with n=3
Would it be possible to perform a pairwise comparison to know about OTU composition related to the respective treatment? in this case, should I consider the average of triplicate samples, for example?
thanks in advance