Mix paired end and single ends sequence in the same analysis


I am comparing different dataset of 16S rRNA, I wondering if I can mix paired end and sigle end datasets in the same analysis.
Thanks in advance

Hello! I do not think you can do that in mothur as mothur needs 2 strands to make error corrections.

If it is really what you need to do, I think your only option is Dada2.

Best of success.