Make.contigs failed as number of forward and reverse sequences are unequal. Please let me know the commands before making contigs at this stage. I got the warning in mothur as
“Processing file pair 18J_S43_L001_R1_001.fastq - 18J_S43_L001_R2_001.fastq (files 1 of 1) <<<<<
[ERROR]: You have 99010 sequences in your forward fastq file, but 247971 sequences in your reverse fastq file. Please use the list.seqs and get.seqs commands to make the files match before proceeding.”
On which fastq do I need to run list.seqs? forward or reverse?
How to remove unpaired sequences?
Please elaborate the commands step-wise before make.contigs command.