how to reassemble the mothur produced file

Commands in mothur
1

Hi,

Is there anybody who can help me? Honestly to thanks in advanced.

I have a set of data which was processed with mothur in 2015. However, I just have these things, some parts missing. raw data is missing.

But I have the file after make.contigs, “.trim.contigs.fasta”,".trim.contigs.summary", “.trim.contigs.summary” exist, while “contigs.groups” missing.

I also have the the files after screen.seqs, “.trim.contigs.good.fasta”(in seperate fasta files, I have 60 samples, so in 60 seperate fasta files), while no “.contigs.good.groups”.

Then I also have the files after unique.seqs ,".trim.contigs.good.names", “.trim.contigs.good.unique.fasta”.

Then I have “.trim.contigs.good.count_table”, “.trim.contigs.good.unique.summary”.

Is there a way in mothur that can rebuilt the data?

Because this is one part of sequencing data, I have two other parts. I want to combine the three sequencing data and processing them together.

2

You really need the raw fastq files. When you go to publish, those fastq files will need to be deposited in SRA.

Regardless… I would probably merge everything based on the output from make.contigs.

Pat