how to make custom reference sequence?

Commands in mothur
1

hello

i want use align.seqs command to align some sequeces to my own set of reference sequences?

howerver, i got error “[ERROR]: template is not aligned, aborting.”

so my question is how to make custom reference sequence ?

thanks :slight_smile:

2

The reference sequences must themselves be aligned to each other. You can pull sequences out of a reference database like we have for the SILVA and greengenes references. Alternatively, you can align protein coding sequences using something like clustal.

Pat