[ERROR] make.contigs() mothur 1.34.4 vs 1.33.2:

[milferst]

Hello,

I’ve been using mothur 1.33.2 on a Ubuntu 12.04.5 LTS computer and am migrating to a new computer using bio-linux 8 (Ubuntu 14.04 LTS). Mothur 1.34.4 came pre-installed with Bio-linux 8.

I executed the command make.contigs(file=fastq.files.table, oligos=miseq.LBE.16S.515_928.oligos.table, pdiffs=0, processors=1) on both computers, using exactly the same input files. Using mothur 1.33.2, the command passes and gives the expected results; using 1.34.4, I get the following error message:

[ERROR]: make.contigs requires paired barcodes and primers. You can set one end to NONE if you are using an index file.

I’ve read the changes that were done on make.contigs() between the two version and noticed that pdiffs are calculated differently now. This does not affect what I am doing as I can increase pdiff to a rediculous number and still get the same error.

Would you be able to tell me what the problem may be and how to solve it?

Best wishes,

Kim

content of fastq.files.table: 3_AN_46_1_glu_sig Tb03_46h_A_glu_GATCTG_L001_R1.fastq Tb03_46h_A_glu_GATCTG_L001_R2.fastq

content of oligo file:
primer GTGYCAGCMGCCGCGGTA CCCCGYCAATTCMTTTRAGT

[westcott]

This is a bug that will be fixed in the next release. The issue is caused because your oligos file does not contain any barcodes. As a workaround add the following line to your oligos file:

primer GTGYCAGCMGCCGCGGTA CCCCGYCAATTCMTTTRAGT
barcode none none myGroup

Mothur will remove the primers, and assign all your sequences to myGroup. You can disregard the group file mothur creates.

[milferst]

Hi Sarah,

Thanks for your quick reply. Adding the line to the oligo file, I am able to run make.contigs(), however, my *trim.contigs.fasta remains empty. On 1.33.2, I obtain a file of the expected size.

Do you have any other suggestion to make make.contigs() add sequences to the contigs file?

Thanks again and best wishes,

Kim

[westcott]

What are the scrap codes you are getting from mothur?

[milferst]

I am not sure what you mean by scrap codes. I obtain only one scrap file for the forward sequence that contains all sequences.

[westcott]

These are mothur’s scrap codes.

k linker missing
b barcode missing
s spacer missing
f forward primer missing
r reverse primer missing
t too many differences to the linker, spacer, barcode, and forward primer
l too short or too long
h homopolymer length too long
n too many ambiguous bases

Also, if a barcode is missing, then you’ll also be missing the forward primer and the spacer if you’ve included one. You can find the codes in the scrap file. The sequence names should look like:

M00704_50_000000000-A3G0K_1_1101_15777_1541 | b fbdiffs=0(noMatch), rbdiffs=7(noMatch) fpdiffs=0(match), rpdiffs=0(match)

You can see from this that the sequence failed because of the barcode. The forward barcode matched, but the reverse barcode had 7 diffs.

[milferst]

Thanks for the codes.

Find below an example for one sequence when using 1.34.4. All others have the same scrap code. I’ve checked again my primer sequences and they seem alright.

HWI-M00185_93_000000000-ABAB4_1_1101_23093_1935 | f fbdiffs=0(match), rbdiffs=0(match) fpdiffs=1000(no Match), rpdiffs=1000(noMatch)

[milferst]

Hi Sarah,

Could you please have another look at my post from Feb 27 where I added the scrap codes. I still have no clue why make.contigs() assigned these high values for fpdiff and rpdiff. As a consequence, I don’t retain any valid contig.

It seems to work just fine in 1.33.2, with only a fraction of sequences being marked as “f” in the scarp file. If I understand right, then you added a lot more information to the scrap files from mothur 1.34 on which probably explains why the scrap code in 1.33. consists only of one letter.

Thanks again for your help!

Kim

[westcott]

The value of 1000 is the default mothur sets. i.e. No primer found yet - The sequence is guilty until proven innocent. It is also used to indicate a sequence that is shorter than the primer. Are you setting the pdiffs parameter? Could you post a few sequences that passed in 1.33.2 and do not in 1.34?

[milferst]

Hi Sarah,

As requested in your last post, I compiled a set of 31 sequences for the forward and reverse files and ran the test data using the command:
mothur “#make.contigs(file=fastq.files.table, oligos=oligos.table, pdiffs=0, processors=3); summary.seqs()”

The content of all the necessary files are added below.

On 1.33.2, the files pass and I obtain a trim.contigs.fasta file that has sequences in it. In 1.34.4 (that came with Biolinux 8), my trim.contigs.fasta file remains empty.

Best wishes,

Kim

content of fastq.files.table:

primer GTGYCAGCMGCCGCGGTA CCCCGYCAATTCMTTTRAGT
barcode none none myGroup

content of oligo.table:

temp01 temp01_R1.fastq temp01_R2.fastq

R1.fastq:

@HWI-M00185:93:000000000-ABAB4:1:1101:14094:1342 1:N:0:GATCTG
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGTGGATATTTAAGTGGGATGTGAAATACTCGGGCTTAACCTGGGTGCTGCATTCCAAACTGGATATCTAGAGTGCAGGAGAGGAAAGGAGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAATACCAGTGGCGAAGGCGACTTTCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTA
+
CCCCCGGGCEEEFGGEFFGGFFGGGGGGGGGGGFGGGGDGFGGGGFGGFFGGGGGECGDGGGGGGGGFFFGFGGGGGGGGGFGEFGGGGGGFCG9AGFFGCEDEEGCF??FEDEFG>FGGGGFGGGFFGGG8FA<FDDGGGFFGCEGCFGGGGGGGGFFGGGGDGFCFG=FFGFGEDC7DEFFFGG83@AEFFGGGGFFFFGGGCGFEGDCCEGEGEGGC77CFFFFCFGGFGGGGFGGGGFGFG=F7?FFGE).:BBB;C6)223<<(72)64CFEFGGG?B9AF9@9?@57<4
@HWI-M00185:93:000000000-ABAB4:1:1101:13950:1388 1:N:0:GATCTG
GTGCCAGCAGCCGCGGTAATACAGGTGTTGCAAGCGTTACTCGGATTTACTGGGTGTAAAGGGTCAGCAGGCGGCTTTGTAAGTCTTAGGTGAAATCCAGCGGCTCAACTGCTGAACGGCCTAAGATACTGCATGGCTAGAGTACGATAGAGGAGAGTGGAACAGCCAGTGTAAAGGTGAAATTTGTGGATATTAGCTGGAACGCCAACGGTGAAGACAGCTCTCTGGATCGATACTGACGCTCAGGGACGAAAGCGTAGGGAGCAAACAGGATTAGATACCCTGGTAGTCTACGCTGTA
+
CCCCCGGGEGGGGGGGGGGGGG-C<CFG@FGGGGGGGGC@,EFGFFGGG<EGGGFFGGGGGGGG8<F@AFFGGGGG:FFGGGGGGFC<FGFGGGGG9FC,B>FFGDGGGGG9BE5B?8FE:F<AFFFGFGGGGGGGGG,ADEFCC>7DF:ECFCC<@7CFFF<,FF<8@<FFG,@FFFGGGFG9,@E8EFFEDFGC87,?>FG7>E7C5FCC5CCFBCEBC<,2>C+74C07/=CFEFFGGB3@F4F?FEGEE4?>5>3E?>BGGGF?3FBFFFFA>0CCF68<?AAFF#########
@HWI-M00185:93:000000000-ABAB4:1:1101:13960:1403 1:N:0:GATCTG
GTGCCAGCAGCCGCGGTAATACAGGTGTTGCAAGCGTTACTCGGATTTACTGGGTGTAAAGGGTCAGCAGGCGGCTTTGTAAGTCTTAGGTGAAATCCAGCGGCTCAACTGCTGAACGGCCTAAGATACTGCATGGCTAGAGTACGATAGAGGAGAGTGGAACAGCTAGTGTAAAGGTGAAATTTGTGGATATTAGCTGGAACGCCAACGGTGAAGACAGCTCTCTGGATCGATACTGACGCTCAGGGACGAAAGCGTAGGGAGCAAACAGGATTAGATACCCTGGTAGTCTACGCTGTA
+
CCCCCGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGCFGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGF<DGGFFGGGGGGGGGAFFGFGGGEFECCG:EFGGGGGFGG;FFGGGGGGGGGCGGGG7F:CGGGGFGGGFF?DGFFGGFGGGGAGFGGFD?55EEFFGF@BFGB>;GGFFFBFFFF?<AFFCGGGCF?B2<F<@F1<7<8A
@HWI-M00185:93:000000000-ABAB4:1:1101:13615:1418 1:N:0:GATCTG
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCAGGCGGTCGGATAAGTCAGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTTGAAACTGTTCGACTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTA
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFFGFGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGCGGGGGAFGGGGGGGGGFFFGGG?FGFFEGGDE5EGGFFGGGGGGGGGFGGCFGGGGGGGGGDGGGGCEGCGGDGGEGGGGFG9?FGGGGC>GGGG>G>C:74@@EEFFDDE50C?E0EC?FBBFF?DFBFFGG8CFF3:>92@<BF?>758:
@HWI-M00185:93:000000000-ABAB4:1:1101:17811:1424 1:N:0:GATCTG
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCAGGCGGTCGGATAAGTCAGATGTGAAAGCCCCGGGCTCAGCCTGGGAACTGCATTTGAAACTGTTCGACTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAAGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGGAGCACACGCCGTA
+
CCCCCFFGGGGGGGGGGGGGGGG;FGGCFFGGGFGGFG@CEGGGGEGEGGGFFGGGGGGGGFG:FFGGGGGDGGFE=7CFFGGGEEGFDGGGGGGF8EGGGGGGEDF<EGGGGG?DEFFFGGG9=A>CFGGFGF>FFBFFFCFFF;FFFGGGC7>BCEEGFGGGGDGCGCFCFFGGG*?EGGFCGGGEEEGCCF?<CC+;@EFFGG5CEEEFGGECEG>8C5:8?>FGGFFCCCEF4<C=D:EBGDF44.7:>375)7>B=0;4=CGFF:F?0<??4AFGGCFC###############
@HWI-M00185:93:000000000-ABAB4:1:1101:14889:1473 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTGTTTAAGTCTGGTGTTTAATACTGGGGCTCAACTCCGGGTCGCACTGGCAACTGGGCAGCTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACCCTCTGGGCTGTAACTGACGCCGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAA
+
CCCCCGGGCFGFGGGGGGGGGGGGGGGEGGGGGGGGGGGGGGGGG@FGGDGGGGGGGGGGGEFFGGGDGGGEGGGDCGFGGGGG9<FCFFG7FFGG,EF9FGGGGDGGGFD>>FEFDGD+>FFF,FFF:FGF>@<BCEB3:<<<<,?<<F:=FC:3<@F@FBGFG@<?9CCEFFF:BCCFAE:;8=C?:79+<+:;5C***;@29EC82C######################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:15272:1481 1:N:0:GATCTG
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCGAAACTGGCAGGCTGGGGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTA
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGFGGGG>CFGGFGGGGGGGGGGGGGGGGGGGGGFGGGGGGGCDFFGGFFGGGGCFC<FGGGFGG5CCFGGGGGDGGGGGGG5EGCEGGGEFGGGGGGG+;C4<F?D7=>FDC68B5CBB?C>DEBG:>;FGDFF2@<2<?BFAC?CGGGGFADAB@464:5C14
@HWI-M00185:93:000000000-ABAB4:1:1101:16912:1483 1:N:0:GATCTG
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTAATAAGTCTGAAGTTAAAGGCAGTGGCTTAACCATTGTTCGCTTTGGAAACTGTTAAACTTGAGTGCAGAAGGGGAGAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGAACACCGGTGGCGAAAGCGGCTCTCTGGTCTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAA
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGEFGGGGGGGGEGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGFFGDGGGGGGGGGGGGGGFGGGGFFGGGGGGGCGGGGGGGFFGGGFGGCDCGGFGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGEFGGGGFGGFGGFFCGFGFGEGDEGEEEGC=EGGGGGECGGGFEGGFGGGFFFAFEGDBE?FFC>BFEEFEEDDFE=6>:CCF53?FF9<?<BFCCEEGGGA9AF##########
@HWI-M00185:93:000000000-ABAB4:1:1101:15548:1501 1:N:0:GATCTG
GTGACAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGGGAGCGTAGGTGGCTGTGCAAGTCTGATGTGAAAACCCGGGGCTTAACCCCGGGACTGCATTGGAAACTGTGCAGCTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTAATGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGTGCAAACAGGATTAGATACCCAGGTAGTCCACGCCGAT
+
C@C6CEFFGGGGEGGGDFGGGEFFGFBFEGCFGDFG-6BCCFF7EGGGGAFGFGC:@@EEFCFFGGE+?FGEEE,CCC@9<EFCFEFGGFFFDFGFGEGEGGGG@F,AFF4CCFGG>FFF=,@>ACFFEFDFG,FFD<FFFGCFFD=FEFGEGG:@FGGGG:,>FFGGGF9DF<@FEGFFFCGCEFGGGGFFF9F@FFGD5FFFFGG80>0/31=:<:58>ECA+A7<:<F99C+<+)<++0?F70:597>BFB:58)85)8(58F7<>::52:)44>2<CE?29458A6C?@?B####
@HWI-M00185:93:000000000-ABAB4:1:1101:15565:1507 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTAATCGGATTTACTGGGCGTAAAGGGTGCGCAGGTGGCTATGTAAGACAGATGTGAAAACCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTGCAACTAGAGTGCAGGAGAGGGAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTCGGAGGAACACCAGTGGCGAAGGCGACTTACTGGACCGGAACTGACACTCAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCC-BFGGGGGGGGGGGGGGGGGGGGGG+@F+FFGCGGG7,CFGG,CFGGGGGG8FGGGGG,C,CFF+CGE<FF4E,EF5EFG<F,BEGGGGGGG9FFG@EGGGG+FFCG:+@F7FGGGGGGG9A,8FGG3,7:5DFGGGG,D,>FGGG1,@FGGCG@FG;,?FEFGGG<FFGGGGCFGGEGGGFGF+<CEGGGGGGGG=CCEEGGD=EG5)/+3<7A6F)12?FBGG9<68+6D/:)8ED3?E>37BG(79CC?78:F?BDFFBBGFCGGGFFAAF:=FFDF3?
@HWI-M00185:93:000000000-ABAB4:1:1101:15548:1520 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCTGTGCAAGTCTGATGTGAAAACCCGGGGCTTAACCCCGGGACTGCATTGGAAACTGTGCAGCTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGTGCAAACAGGATTAGATACCATGGTAGTCCACGCCGTA
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCFGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGFGGGGFGGGGGGFFGGGGGGGGGGC;BDFGGGGGGGGGGGGGFGGG5=EFGGGGGGGGGGGGGGGGGFGDGEEEEGGGGGGGE99:>EDFGCEFBFEGGFGFFGGF?EFF>:?EB>35E;DFF?CFF?FBFDFF<>C0<+<766449<@=3)>CFF
@HWI-M00185:93:000000000-ABAB4:1:1101:8318:1530 1:N:0:GATCTG
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTGGATAAGTTAGATGTGAAAGCCCCGGGCTCAACCTGGGAATTGCATTTAAAACTGTCCAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCCCCGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGFGGGGGGGFGEFFGGGGGGGGGGGGGGFGFGGGGGGGGGGGGGGGGGGGGGFFFGGGDGGGGGGGGGGGGGGGGGGGEEGGGGGGGGGGGGGGGGGGGGEGG@EGFGGGGGGGGCGGGGGCEEGGGGGGGGGGGGGGCGGGGGGGGGGGGGFGGGGGGG8FGGGDGFGGGG>E>EE>G6FFB:FF>47?AAC9<6CFF:DFFFGF?:D5D>?
@HWI-M00185:93:000000000-ABAB4:1:1101:16728:1543 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTATATAAGTCAGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTTGAAACTGTGTAACTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATTTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAATGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGCCCACGCCGTA
+
CCCCCGGF,CDGGGGGGGGGGGGCEEGGGGGGGGGGGG@@FGGDGGGGG<?EFGGGGGGGG<F<FGGGGGFGGGGGGGGADFGGGGGFFFGGGGGGDFGGGGGGGGGGFFCF@@DGGGCE:CECEGGGGGG?:CEFGGGGGGGGGGGGGDGEEEGFGG+<CFG88;CGGGGGEEGGGGGGGEGG5C>8C>8@FG+++3<<4<FCDD57?E><?FFGFFFGDDFFG?F>FFGFBFF::A<EGGGCFGBAA7?CF:15>DB?
@HWI-M00185:93:000000000-ABAB4:1:1101:8326:1549 1:N:0:GATCTG
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTGGATAAGTTAGATGTCAAAGCACCGGGCTCAACCTGGAAATGGCATTTAAAACTGTAGAGCTAGAGTATTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGCCCCCCTGGACAATAACTGACGCTCAGGGACGAAAGCTAGGGGAGCAAATGGGATTAGATACCCTGGTAGTCCTACCCGTA
+
CCCCCFFFGGGGCGGFFGGGGGGGGGG+BFFFGCFGFGE@<EFF@CDFF<FG8CFFC@CFFGG7FGGGEFG7FGB:4+9,<CFF,55C,@E,@ADF=,B:CGDCFFFFGG,B,BF<,CFBB>,BFFFCF<,:BFFGGGGG,E,@CFG9FF+@**/2<CFFGGGFCGGFGGGG2?CEAFEFC/CEGGFFC:F@C6;CF6@++/8?C==GGE5*@##################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:20974:1567 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGTGGATATTTAAGTGGGATGTGAAATACTCGGGCTTAACCTGGGTGCTGCATTCCAAACTGGATATCTAGAGTGCAGGAGAGGAAAGGAGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAATACCAGTGGCGAAGGCGCCTTTCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCCCCGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGCFGGGGGGGGGGGGGGGGFGGGGGGGGDFDFEFEEGGGGGGGGFFGGGGGCBFGGGFGGGGGGGGGFF<DF=FGGGGGGDFGGGGGGGGFFFGGGGGGGEFFGGGGGGGGGGGGGGFGG5>GGGGGEGGGGFGGGGGGGGGGGEGGGGGGGGCGGGGGGGGFGGFFGGGGFGGGGFGFFGGGFB:EEFFGFFF@GBFFGDGFFFFF228?BFGGFGGGG?AAAF<?FFFFG><
@HWI-M00185:93:000000000-ABAB4:1:1101:18671:1605 1:N:0:GATCTG
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGGCTGGTAAGTCAGCTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTTAGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCAATTGCGAAGGCAGCTGACTAGACTGTTACTGACACTGATGCTCGAAAGTGTGGGTATCAAACAGGATTAGATACCCTGGTAGTCCACACAGTA
+
CCCCCGGGCFGGGGGGGGGGGGGGGCGE@FG@CFEFGGGGGGFGGF7DFFFFGGCFGFEGGGGGGGFFFEGCFGGGFEGGGFGGFFF?FFGGGGGFCE,FFFGGGGGGGGGDFCFEFFFGGFFFACFFGGF9,CFFFGBFFFF9F@BCFGGGG8FFG9FEFEFGGGC*>FFFGGGEGFGCFFGGGF9BFGG@CFG@EEEGGGGCGFFFFCGGEGG5:CCEDCF9AGEFGGGF7EF<CAGGFGFF<+3C7<@>FEE9@@?37:4)6CC@016?A?FAAC+A8CF58>>B=<6F07><A<
@HWI-M00185:93:000000000-ABAB4:1:1101:14042:1612 1:N:0:GATCTG
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGTGGATATTTAAGTGGGATGTGAAATACTCGGGCTTAACCTGGGTGCTGCATTCCAAACTGGATATCTAGAGTGCAGGAGAGGAAAGGAGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAATACCAGTGGCGAAGGCGCCTTTCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTCGAGACCCTGGTAGTCCACGCCGTA
+
CC<CC9C@FGGGGGG+FEG9<F@FF<CGGG<-EFGE@;@FE<CE7@@F<9@AFGFFGEG@FFEEGDC@FF@6CFFFDFFFFDFCFDDFFGGFA<<EEE,CF+4:F8FGGGGGGF87B<ECEFDFFF,:=FFF,7EFFFFGGGFG<DF+>DEGGFCFGGFGECB,CGGFGGGCGGGE779>AFGG8CEC9@FFGGAD9D83><<,=DDF?ECCEG7FDEG@<99C9F<;?CF+>99)+=A6)<AFGGF=D3075))83B>F##################################
@HWI-M00185:93:000000000-ABAB4:1:1101:11122:1613 1:N:0:GATCTG
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTATATAAGTCAGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTTGAAACTGTGTAACTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCCCCGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCFGGGGGFGGGGGGGFGGGGGGGEGGGGEEEGGGGGGGGGGGGGGG9FEFGGGGGGGGGGGGGGGGGGGGGFGGGGGGGG>>FEEEGFGGGGGGGGGFFFGGGGGGGGGGGDGGGGGFCFFGGGGGGGGGGGGGGGGGGGGGGGEEG5CEGGGGGGGGF6+;<4CGGGFDFGGFDDFBEFFFG@3;5:6@1FGGFF2><?A772:EFCGGGGF<2<@<FFFDFD;3
@HWI-M00185:93:000000000-ABAB4:1:1101:18686:1615 1:N:0:GATCTG
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGGCTGGTAAGTCAGCTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTTAGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCAATTGCGAAGGCAGCTGACTAGACTGTTACTGACACTGATGCTCGAAAGTGTGGGTATCAAACAGGATTAGATACCCTGGTAGTCCACACAGTA
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGFGGGGGGGGGGGGGGGGGGGGGGGFGGGGEFFGGGGFGGGGGGGFFBFFGGCGGGGGGGGGGGGGGGFGFGGGGGGGGGFGGFFGGGGGGGGGGGGGGGGFGDFFFFFGGGGGFGGGGFBCGGGGFGFGGCFGFGFGGGFCEGFFAFGGGGGGGGGGGGCFGGFGEGE=GGGGGGGGGGG7FGGGFFGGGAFGFF494<FG*-07ABFFGFFDEG<46EFFFFF5()-4366<C?FGGFF9:4@GF?:@FC<<
@HWI-M00185:93:000000000-ABAB4:1:1101:14064:1617 1:N:0:GATCTG
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGTGGATATTTAAGTGGGATGTGAAATACTCGGGCTTAACCTGGGTGCTGCATTCCAAACTGGATATCTAGAGTGCAGGAGAGGAAAGGAGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAATACCAGTGGCGAAGGCGCCTTTCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACCGGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGC@FGGGGGGGGGGGGGGGGGGGGGGDGGGGGGFGGGGGGGGGGGFFFEGGGGGGGFCFFGFGGGGCFEGGGGGGFFGGGEABFGGGGGGGFEAFFGFFGGGGFDGEGGGFGGGGGCGGGGGDGFCFDGGGGGGGFGEFDGGGGGGGGGG8CEECFEFFGGGGBECDFGGGGGGGC::::B588<FGFCF9B+CEGGGGG97<6C4)0)>:FF;37C<35?7557C:>>GGF)((0((,4265<9ECCF:(.52=E461>FD##
@HWI-M00185:93:000000000-ABAB4:1:1101:15060:1626 1:N:0:GATCTG
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGTGGATATTTAAGTGGGATGTGAAATACTCGGGCTTAACCTGGGTGCTGCATTCCAAACTGGATATCTAGAGTGCAGGAGAGGAAAGGAGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAATACCAGTGGCGAAGGCGCCTTTCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCCCCGGGGGGGEGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGFGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGFGFGGGGGGDFGGGGGGGGGGGGGGGGGEGGFGGG9EFGFFFFG8,DACFDCEEFFGGGGDGGFGFGGGGGGGGC<EGGGFGFGDGF;>BGB>F>BDE38386CEEF<?568<2>DAFCCFGFD:>BAFFFF?DFD##
@HWI-M00185:93:000000000-ABAB4:1:1101:9832:1639 1:N:0:GATCTG
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGAATAACTGGGCGTAAAGCGAACGCAGGCGGATAGATAAGTAAGATGTGAAAGACCCGGGCTCAACCTGGGAACTGCATTTGAAGCTGTCTGACTAGAGTCCAGTAGAGGGGAGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCAGTGGCGAAGGCGACCCTCTGGACAGTGACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGGCCAAGACGTA
+
CCC-CFGGGGGGGGGDGGGGGGG@CFG76BFGFFFGGG,CFCE:C6@FGEGGGCECEFG+@C7@FD4@C+=FE,FBFGF55CFEEFFFFFA,?,=FGGGE<FEFFFGGG,DEGGGGA,5BE9>F@,@,:DFFFGGF<,@8EFGGGG++5:BFFFAFGE3,@<DCGFB:C:FCGEGFFE5CGE,@,7?F@CBCGF,C+<A9?EGEGCGG798212<<GG######################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:23613:1647 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATGACCGGGCGTAAAGCGCACGCAGGCGGTCGGATAAGTCAGATGTGAAAGCCCCAGGCTCAACCTGGGAACTGCATTTGAAACTGTTCGACTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCAAGCCGTA
+
CCCCCGGGFGGGGGGGGGGGGGGGEGG:FFGGGGGGGGGCCFGGGGGGGGDFGGGGGGGGGGDCGGGGGCFGGGGGGG7FBEFFGEFF9FFFGGGGE<+CC+DFFFFFGG,:B+BF<<<FF9F9FGFFFGCC,@BFGE@CGFC=,7:FFGA;F1>888EEFFGGF++;FGGFFGGGGGGGFG7C?ECEGGGF?99CG6EGGGGEG5:CCCGGEC5@C:7:5EEGDEGGGGFC?EG06E5.:1EC703<7E>);80<@):0>6CFG52083)6<5<BGC@##################
@HWI-M00185:93:000000000-ABAB4:1:1101:20000:1651 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCAGATAAGTCAGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTTGAAACTGTCTGACTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTA
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGCCFGGGGCCEGGEGFGGGGGGDGCGF@FGGGGGGGEFGFGGEGEGEGGE8CEGGGGGGGEEGGGGGGFG6FC?GGGG576@GG48BB?@D4CEDEFG?:6FGGFBFFFFB?<<2+AFGFFGFFFFF7*?855<?A
@HWI-M00185:93:000000000-ABAB4:1:1101:10499:1656 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTGGATAAGTTAGATGTGAAAGCCCCGGGCTCAACCTGGGAATTGCATTTAAAACTGTCCAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTCGTCCACGCCGTA
+
CCCCCGGGGGGFGGGGGGGGGGGGFGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGDGGFGCFGGGGFGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGFGGGGGGGGGGGEGGGGGGGGGGF:<@GGGGGGGGGGGFFF?GGGGG=<EE@FGGGGGGGGGGGGGGGGGGGGGGGGEGEGEGGGGGGGFCCFFGFGGGGFGFGGG4?F>GEBFGFBBBGFFBGGCFBFF()6<?ADC:CGGGG?)(48;>4BFD>0
@HWI-M00185:93:000000000-ABAB4:1:1101:9825:1657 1:N:0:GATCTG
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCAGGCGGTTAGATAAGTCAGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTTGAAGCTGTCTGACTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAGAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCCCCGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEFGGGGGGGGGGGGGGGGGFGGGGGGGGGFGGGGGGGGGGGGGDGGGGGGGGGGGGGFGGGGGGGGFFGFGDGGGGGGGGGGGGGGGGGGGGGCCGGGGGGGG5CFGFFGGEEEGGEFGFCEGGGEGGGG@CGEECFGEEGEGGGGGDEEECCCFFGG7CEC?FGGG@C>@CCFBFBEEFFGD@BBEBD2EFE@:<F?>>FFAA:+3E6C7:<<A=<@??DFGFF
@HWI-M00185:93:000000000-ABAB4:1:1101:20014:1662 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCAGATAAGTCAGATGTGAAAGCCCCGGGCTCAACCTGGGAACTACATTTGAAACTGTCTAACTAGAGTCTAGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACTAAGACTGACCATCAGGTGCGAAAGCGTGGGGAGCAACAAGGATTAGCTACCCTGGTAGTCCCAGATGTA
+
CCCCCGGGFFGGEGGGGGGGGGG7FEG7FEGGGGGGGG7,6EFGGGGGFGAAFFGGGGGGGFGD7CFEFGGGFG=48,CFAFFFFGGGGGGFGG9BDFFFE@FGGGC<3B3>8>,BFGG9EFEGGGG,37,8<>AFGFC,=,@EF=F,3@FGGGGFFGG;9FGGGGFGEGG:CGFFCFECCEG?ECFFFCF@EEFGGGG*::CGGGGGEEFGCD>8?CEC*@D++3<FG:CC+0+,<F:925<EE)7:;35>G<F9CG)38).,-54(-<CFGFDD##############
@HWI-M00185:93:000000000-ABAB4:1:1101:20140:1666 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAGTACGAAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGCTTTATAAGTTAGGTGTGAAAGCCCTGGGCTCAACCCAGGAACTGCACTTAATACTGTAAAGCTAGAGTGAGATAGAGGATAGTGGAATTCCTAGTGTAGAAGTGAAATTCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTATCTGGGTCTCAACTGACGCTAAGGCGCGAAAGCATGGGGAGCAAAAAGGATTAGATACCCTGGTAGTCCATGCCCTA
+
CCCCCGGGGGGGGGGGGGGGGFGGGGGGGEGGGFGGGGEFGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGEGGGGGFGGGGEFGGFGGGGFGGGGGGGGGGGGGGGGGGGFGFGGGGGD8DGCFGGGGGGGGGGGGGGCFGGD@@FFGCFFGGGGFGCFFFFFFFGFFFGG@FFFF9EFFF9DFEFFGCFGGGGGGGGEGGF7EGEGGGGGGCEC5CEGC?:<?@FGDCG?FCC::FCGGFGFGFGGFFFE>DFGFB5>E:EFC<)48>B>FF?F<F9CG4AC7:42B<FFAF?###
@HWI-M00185:93:000000000-ABAB4:1:1101:15007:1668 1:N:0:GATCTG
GTGCCAGCAGCCGCGGTACTACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCAGGCGGTTATATAAGTCAGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTTGAAACTGTGTAACTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTGGCGGTGAAATGGGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTA
+
CCCCCGFFGGGGGGCCFEEFGGED7FBFFE7EAF?F@@FFGGGD@FFEGFGGGFFDGG:CFFEGEFGGGGGGGGBFGGFGCCEB9?AFFFF<F<9FGEDEEGGGCFAEBFGGCE<8AAF9DF<?DEE8CCFGGFGGGCCC6CEEFGGEECGD+<+;0:EFF9CEFFF+;C58<C0@CECC5FDGCE88CGGGGE=8CC8C6FFFFGG3:3/.>:8)075E?B6=EAF:1),.6).-.:9CFFFG35:<=C@=8B####
@HWI-M00185:93:000000000-ABAB4:1:1101:10515:1673 1:N:0:GATCTG
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTGGATAAGTTAGATGTGAAAGCCCCGGGCTCAACCTGGGAATTGCATTTAAAACTGTCCAGCTAGAGTCTTGTAGAGGTGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAAGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCCCCGGGGGGGGGECFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG>CEGGDG@CFGG@FGGFFGGGFCFFGGGGGGGGGGGGGE7+CBFGGGGGCGGCF<EGGGFGGFGGGGGGGFAAEG7FFGGFGE,=BFFGGG,3*@CBCFCCFFGFGGGGGGGGGGGGGGFGFGGEGGC7;CFF99?F>ECFGGFE=ECCFGGDC88:<8:EGGEEGGG/:CC89)367;:4FCFC77@G:>FE>EEBGF33:0<>:(.42<9255++3CFGG().8BC=F6BFDB?
@HWI-M00185:93:000000000-ABAB4:1:1101:10167:1692 1:N:0:GATCTG
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGTGGATATTTAAGTGGGATGTGAAATACTCGGGCTTAACCTGGGTGCTGCATTCCAAACTGGATATCTAGAGTGCAGGAGAGGAAAGTAGAATTCCTAGCGTAGCGGTGAAATGCGTAGAGATTAGGAAGAATACCAGTGGCGAAGGCGACTTTCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTA
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFEGGGGGGGFGGGFGGGGGGGGGFFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFEGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGDGGGGGG@FGGGGGGGGGCGGGFGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGGGGGGGGGGGFGGGGFFAGGGGFFFFEFFGDE@>E<EBF;CFB0:??B?FA>GCFG=FD2)6AFFFF(75C##

R2.fastq

@HWI-M00185:93:000000000-ABAB4:1:1101:14094:1342 2:N:0:GATCTG
CCCCGTCAATTCCTTTAAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCGGAGCCCACGCCCAATGGCCCAGCCTCCAAGTTGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCAAGCTTTCCAGCCCCAGTGTCCGTTACAGTCCAAAAAGGCGCCCTCGCCACTGGCATTCTTCCTAATTTCTACGCATTTCACCGCTACACTCGGAATTCTACTTTCCTTCCCCGCACTCTATATATCACGTTTGGAATTCAGCAA
+
6ACCC@@BBFAF,CFFGFGGGGFAEDFGGGFGEGECFFCF@FGECGG@FGF+B<F9FFGFFFGGGGGGG,CFC:@F7=D=F79=,9C,:,B+8,+,A,=FG99?E,=FC8F+,58A+?+6=4>A8C,AFE3,732,@D@:,>@F9,82+6=6@+,+2+@5+2,@8+@5CC###############################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:13950:1388 2:N:0:GATCTG
CCCCGCCAATTCCTTTGAGTTTTAGCCTTGCGACCGTAGTCCCCAGGTGAGTCACTTAACGCGTTCGCTTCGCCGCCCAAATCCGAAGACCCAGACCGCCAGTGACCATCGTTCACAGCGTAGACTACCAGGGTATCTAATCCTGTTTGCTCCCTACGCTTTCGTCCCTGAGCGTCAGTATCGATCCAGAGAGCTGTCTTCTCTCTTGGCGTTCAAGCTAATATCCACATATTTCCCCTTTACACTCGATGTTCCACTCTCCTCTCTCGTACTCTAGCCATGCAGTACATTAGACCGTTC
+
CCCCC7FGGGGG<FGGGGGGGG9E@FEGGGGDFCFGGGGGGGGGGGGFGE,C,;FGFGAFGGFGC:CFFGCFGGGG7+444<CF:FGCB,BFG8FB+C+CC7=F<FECDDCGG,B95F<CFGE7,>,D3A@FCFDFGFFF8>:>,DD,:E,AD6<@F9>C*@DDCG7C,0@>C>2:,;9CG:EF@CG:C/8;C#######################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:13960:1403 2:N:0:GATCTG
CCCCGCCAATTCCTTTGAGTTTTAGCCTTGCGACCGTAGTCCCCAGGTGAGTCACTTAACGCGTTAGCTTCGCCGCCCAAATCCGAAGATCCAGACAGCCAGTGACCATCGTTTACAGCGTAGACTACCAGGGTATCTAATCCTGTTTGCTCCCTACGCTTTCGTCCCTGAGCGTCAGTATCGATCCAGAGAGCTGTCTTCACCGTTGGCGTTCCAGCTAATATCCACAAATTTCACCTTTACACTAGCTGTTCCACTCTCCGCTATCGTACTCTAGCCATGCATTATCTTCGGCCGTTC
+
CCCCCGGGGGGGGGGGGGGGFGGGGGGGFGGEGGGGGFFFGGGGGGGFFGCGFGGGGGGGGGGGGDGGGFGGGGGGGGGDFGFFGGGGGGGGFFFGGFDGGGFGGGFGFCEGFGGGGFGCFECFGGGGFGGGGGGFGFGFGGG=EFGFFFGGGGGGGGGDC@CGCGCEFGA?3<?B>AFAE5:<>C97DFGGGFGFC@?8B?:95B:@DFFG;?).;>;C+??<E@E;;F####################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:13615:1418 2:N:0:GATCTG
CCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCGGAGCCCACGCTCAATGGCACAGACTCCAAGTTGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCCACGCATTTCACCGCTCCACCTGGAATTCTACCCCCCTCTACAACACTCTAGTCGAACAGTTTCAAATGCAGTTC
+
CCCCCGG@FGGGGGGGGGGEGGGGGGGGGGGGGGGGGFGGGGGGFGGGGGGGGGGGGGGGGGEFGGGGGGGGGGGGGGGDEGGEGGGGGGGGGGGGFFGGGGGGGGFFGGGGGGGGGGGGGGGGGGFGGGGGGGGGG7EFGGGGGFG9@FCCDFGGFGGC>@ADGDEEGDFGGBDFDD:FFEGGFGFC@;FGGGDGGGE@D?DGDFD>C3>EGB>A8?B<6644)5((938?4C#################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:17811:1424 2:N:0:GATCTG
CCCCGTCAATTCCTTTAAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCGGAGCCCACGCTCAATGGCCCAGACCCCAAGTTGACCTCGTTTACAGCGTGGACTACCAGGGTATCTAAACCTGTTTGCCCCCCCCGCCTTCGCACCTGCACGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGCACACTACGCATTTCACCGCACCCCCTGGAATTCTACCCCCCTCTTACAGCCTCTAGACGAACAGTTTCAAAACCAGTTC
+
BCCCC@FGGGGGGGGGDGGGGGGGGGGGGDFG7CEGFGGFGGGGGGGFGE@FEEGFGG,9CEGGEG:FFAFGG:FG@FG,C7,48?,5=E,B=8:4+<C=FF<E<EC9DFEFFGGC?,56@E7?==A>>,8B>==E9@D,3=88FFG,6,+=CCC9C;15*;;EG80),;=/)2)9+5/+2:8+9CFEDDD95<,4DE?58C###########################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:14889:1473 2:N:0:GATCTG
CCCTGTCAATTCCTTTGAGTTTCAGCCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATGTGTTAACTTCGGCACCAAGGGTATCAAAACCCCTAACACCTAGCATTCATCGTTTACGCCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACCCCCCAGCGTCAGTTACAGCCAAGAGAGTCCCCTTGGCCATCGGCGTTCCTCCACACCCTCACGCATTTCACCCCACACCGTGGAATTCCACTCCCCTCCTCTGCACCCAAGCTCCCCCGTTTCACGTCAGCA
+
ACC@CCFGGGGGGGGG9EFEGGFGEAFGGGGG7FGGGGGFGGGGGGGGGGGGGGGGGGGGG<FFFGCF<@FGGGGGD<FFGCCEFE,9EFGGGGGG,AF44<<,AF,5499=FGC+9@+4+8CCEF?8<,8?7D=6=E9,@C,5FFF8DFGGF71++0@@?########################################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:15272:1481 2:N:0:GATCTG
CCCCGTCAATTCCTTTAAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACTCCTCAAGGGAACAACCTCCAAGTCGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACCCCTGGAATTCTACCCCCCTCTACAAGACCCCAGCCTGCCAGTTTCGAATGCAGTT
+
ACCCCGGGGGGGGGGGGGGEFGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGEGGGGGGGGGGFGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFGGGGGGGDE@CGGGGGGGGGFGGGGGGGGGFGGGGGGGFGGGGGGGGFGFFGGGGGGGGGGGGGFGGEDCF4CDFDFFFFFF?FGGFFF9CFEGGG:D<53<;CEFFG5C>587<B<<(3:097)64AD3><:BGFFFFF99(((.>??<9F?A)>1(-244:))08(.43(4?F?FF7><FD)-49C<6@D9
@HWI-M00185:93:000000000-ABAB4:1:1101:16912:1483 2:N:0:GATCTG
CCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCGGCACTAAGCCCCGGAAAGGGCCTAACACCTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGTGTTCCTCCATATATCTACGCATTTCACCGCTACACATGGAATTCCACTCTCCCCTTCTGCACTCAAGTTTAACAGTTTCCAAAGCAAA
+
CCCCCGGGGGGGGGGGGGGFFGGGGGGGGGGGGGGGGGFFGGGGGGGGGDGGGGGGGGGGGGGGEGEGGFGGGGGGGGGGGGGGGGGGGFGGGDGGDGGGGDGGGGGDGGGGFGGGF;CEGGGGGEFGGGGFD77@>DFFDDDCFFEFGGGGGCGFGDGGDEAFGEGCCGFGGDFDEB9CC?GGFGGGGGF:,:5<<:67>DEDAEDDEFFC?AA<9:09<4)5)60(.1:96A9<.3>916<F??9C9@2)5:4FCF#######################################
@HWI-M00185:93:000000000-ABAB4:1:1101:15548:1501 2:N:0:GATCTG
CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGGAATACTTATTGCGTTTGCTGCGGCACCGAAAAGCTTTAGCTTCACGACACATAGTATTCATCGTTTACGGCGTGGACTACCAGGGTATCTACTCCTGTTTGCACCCCACGCTTTCGAGTCTCAGTGTCAGTTAAAGTCCAGTAAGCCGCCTTCGCCACTGGTGTTACTCCTAATCTCTGCGTATTTCACCGGCACACTAGGAATTCCAATTCCCTCTCCTGACTGCAAGCTGCAACGTTTCCAATGCCGT
+
ACCCCGGGGGGGGGGGFGGGGGGGFDFGGGGGGGGGGGGGGFGGGGGGFGCFGCFGGCFFCCCFECGGGGGGEDEEDFGG+CFFAFFEFFGGG,FEE7FG,BEFGFEC9>FFGG@F<AF==@FCEGGFGGFFD7BFDD,==,6DFGGGFD++=F@F88+=EGGGGCE9,=F@DCB11ACD9,+35,=;CFCEGCDC45):=:AG,?##########################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:15565:1507 2:N:0:GATCTG
CCCCGTCAATTCCTTTGAGTTTCAACCTTGCGACCGTACTCCCCAGGCGGACTACTTATCGCGTTTGCTGCGTCACTGAACAGCAATCGCTCCAACACCCATATTATACCGCGTTTACGGCGTGGTATCACAGGGTCTAAAACCCTTTTTTCACCCCACGCTCTCGAGCCACAGTCAGTGTTACCCCCCAGGAAGCCGCCTTCACTCCTGGTTTCCCCCCATATCTCCGCGCTTTTCTCCGCCACACGAGGATCTACACCCCTCTCTCCCGCTCTATACATGCACTGCACCATACGCATT
+
CCCCCGGGGGGGDGGGGGGGGGGG9-CEFGGG@:FGGGGGGGGGGGG,@C+7+BFGGA,CFGGG:CFG@FG,+BF,CFG,EFG########################################################################################################################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:15548:1520 2:N:0:GATCTG
CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGGAATACTTATTGCGTTTGCTGCGGCACCGAAAAGCTTTAGCTTCCCGACACCTAGTATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCACCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATAACTACGCATTTCACCGCTACACTAGGAATTCCACTTACCTCCTCTGCACTCTAACTGCCACTTTTCCAATGCCGT
+
CCCCCGGGGGGGGGGGG9FFGGGGGGGGGGGGGGGGGGGGGFFGGGGGGGGGGGGGGGGGAFGGGGCFGGGGGGGGGGGGGGGGGFGGGGGGGGGD@FGGGCGGGGGGFGCFGGGGGGDEGFGGGGGFF@EFGGGGCDFGFFFFGGGGGGCDFGGGGGGDDECFGGGFGGFGG?FFFCEG7AGGGFGGGGGGGFGGGGD?=?3CE>;E,6;F@;;CE<>@;CA>9).)/2()1;D<).9=71144,?22=FFF::9A###########################################
@HWI-M00185:93:000000000-ABAB4:1:1101:8318:1530 2:N:0:GATCTG
CCCCGTCAATTCCTTTAAGTTTTAACCTTGCGGCTGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCGGAAGCCACGTCTCAAGGACACAGCCTCCAAATCGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATCTCACCGCTACACCTGGAATTCTACCCCCCTCTACAAGACTCTAGCTGGACAGTTTTAAATGCAATT
+
CCCCCGGGGGGGGGGGGGGGGFFGGGGGGGGGGGEGGGGGGGGGGGGGGGGGG:CFGGGGGGGGEGGGGGGGDGEEGGGGCFGGGFDDFFGGFGFGGGGGGFFGGGGGGEFGEGGGGGFGFGDBAE@=<4CFGGGEF?4CGGGGGG3)6@<C8;DECC>DBGBBA4?:2>??AAAAA<;;:(4:?(2715???::79,6>2<FFFF908<AFFF<?<))…:><<7<<0:>?F<<G2<4@6<D
@HWI-M00185:93:000000000-ABAB4:1:1101:16728:1543 2:N:0:GATCTG
CCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCGACGCCCACGCCCTATGGCCCAGACTTCCAGTTGACATCGTTTACGGCGTGGGCTACCAGGGTATCCAATCCTGTTTGCTTCCCCCGCTTTCGCACCTGAGCGTCAGTTTATGTCCCGGGGGCCGCCTTCGGCCCCGGTATTCCTCCCGATCTTCACGGATTTCACCCCTACACCTGGAATTCTACCCCCCTCTCCATGACTCTAATTCCACCGTTTCAAATGCAGTTC
+
CCCCCGEGGGGGGGGGGGGGGGGGGFGGGFFF@FGGGGGGGGGGGGGGGGC+6CFGGGCAFGGGGGGGGAFED,+>==FCF7C7@BA?B=,FC8,+,5:,9A?F<,4,+4BBAE:F@8>6CG?,3>F,8BFG,8+,@DBC>,8FFCA7+?+@#################################################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:8326:1549 2:N:0:GATCTG
CCCCGTCAATTCCTTTAAGTTTTAACCTTGCGCCCGTACTCCCCAGGCGGTCTATTTAACGCGTTAGCTCCGGAAGCCACCGCGCAAGTACACAGCCTCCAAATCCACATCGGTTACAGCCTGGACTACCAGGGTAACTAATCCTGTTTGCCTCCCCTACTTTCGCTCCTGAACGTCAGTCTTTGTCCAGGGGGCGCCCTTAGCCACCCGGATTTCTCCATAACTCTAAGCTATTACCCGCTACACCTGGGAATCCACCCCCCTCTAAAAACACCTACCTTGACATTTTTAAATGCACTT
+
CCCCCCGFGGFGFFGG<FFGEEC@F-FFFGGF+8+8CFGGGGGGGFFGEG77,C,CFDFFGGGGGGFGF,CFGCC+C,EE,+++@C++,:CE,>9FE4EFFBGA<4,@E@,+4BF+5,C8+4<D,56AF:,BB33,037@83E,C>,=2+=>B+6+02@C+43+@:@##################################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:20974:1567 2:N:0:GATCTG
CCCCGTCAATTCCTTTAAGTTTTGATCTTGCGACCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCGGCGGCACAGAGGTCATGACAACCCCTACACCTAGTATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTTCTCCCCACGCTTTCGAGCCTCAGGGTCAGTTACAGTCCAGAAAGGCGCCTTCCCCACTGGTATTCTTCCCAATCTCTACCGCTTTCAACGCTACACTAGGAATTCTCCTTTCCTCTCCTTCACTCTAAATATCCCATTTGGAATGCACCC
+
ACCCCGGGGGGGGGGGGGGGGGG8CFFGGGGGGGGGGGGGGGGGGGGGGGGDFGGGGGEGGGGGGGEG@BF@EEGGGAGFGGC,@?F,FGGGGGGGG@AFGGEADEAE9DE<CFGGGCCC+F+F@FFGGGFGGGCGCBEF73<EF=FGG7B7?FGF:;9;;DF>EFGECFG4,6;A6=89?CGGFC<=**FFCDD2)9.37(0<@B>6B8+9C?#####################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:18671:1605 2:N:0:GATCTG
CCCCGCCAATTCATTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGGCCGCTTACTGTATATCGCAAACAGCGAGTATTCATCGTTTACTGTGTGGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTCAGCTGCCTTCGCAATTGGAGTTCTTCGTTATATCTAAGCATTTCACCGCTACACCACGAATTCCTCTAACCTCTACTGTACTCAACACTGACAGTATCAACTGAAACTTTA
+
BCCCCGGGGGGGGGGGGGGGGGFGFFGGGGGCCFEEGGGGGGGGGGGDGGGGGGGGGGGFGEGGGDGGGFFFEGEFGGGECGGGFG,CFC=FGGGEGDGEGG9D9FGFFB=FBFG9:E5,?:F9AE?FFCGGF8?;A1=,CD,>FFDFFGCF8FFG8FFFE6+++,@@69,2@DFFFEF7>E6=;D@;FF@FEFF5;C559A>E=AB,6?#########################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:14042:1612 2:N:0:GATCTG
TCCCGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCGGCTGCACAGAGGTCATGACAACCCCTACACCGAGTATTCATCGTTTAAGGCGTGGACTACCATGGTATCTAATCCTGTTTGCTCACCACGCTTTCGAGCCTCAGTGTCAGTTACCGTCCAGAAAGGCGCCTTCGCCCCTGGTATTCTTCCTACTCTCTACGCCTTTCCCCGCTACCATAGGAATTCTCCTTTCCTATCCTGCACTCTAGCTAACCAGTTTGGCATTCAGCC
+
-A8<BE;68E<CFCCFECCCF9CAFFGG<6C@@@@F@FFDFFCECF<CF6:FGCGFFCCC<CG@@BC<@CG+8B,9C<?8@F<<9EFFG9==FED<D8E<+@:=<F9E?CFEGFG7,A::+:,A8FGD,CFGF,=3E,CD;;95FF,72A+CF=FDG+=::CGGG6,43E=1++;5,=,9@@BC:<289*//0(0(9(28+.1/7<?EFF;5@EC93:.8DGE);D>FB(9()5?DF6?:<<9<F7@#######################################
@HWI-M00185:93:000000000-ABAB4:1:1101:11122:1613 2:N:0:GATCTG
CCCCGTCAATTCCTTTAAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCGAAGCCCACGCTCTATGGCACAGACTTCAAGTTGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCCCCGGCATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACACCCCTCTACAAGACTCTAGTTACACAGTTTCAAATACACTTC
+
CCCCCGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGDFGGGEFGEGGGGGFGFEGGGD@EFFGFFGCFDGGGGG>FFGAEFGFGGGGGDDGFFGGFAF=EF=CFGGGGEEGGGDGGGGGFGDGGGFDBFFGDDFEGGFGGDEGGGGGCFCGG67AD6EEECFFGGGGF>D/BFEG72>;<;>DC<:CD)7C)7(:7=7>>E/5>>76<AB##################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:18686:1615 2:N:0:GATCTG
CCCCGCCAATTCATTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGGCCGCTTACTGTATATCGCAAACAGCGAGTATTCATCGTTTACTGTGTGGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTCAGCTGCCTTCGCAATTGGAGTTCTTCGTGATATCTAAGCATTTCACCGCTACACCACGAATTCCGCTAACCTCTACTGTACTCAAGACTGACAGTATCAAATGCAATTTTA
+
CCCCCGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGFFGGGGGGGGGGGGGGGGGGGGGGGGGDGGEGGGGGGGGGGGGGGGGGGGGGGGCFGGGGGGGGGGGGFDFGDGGF,CECEFFFFGGFFGGFFGGGFGGGGGGGGCFFGGFGFGGGFCDDCFFGGGGDCFACFDFFFAFGEFGGG:EFFGGFGGGGGGFFF4CF;96?AFFGCFB2>27@@<?EFBB8@@?CFB@F>FFFF@0?B@FCDFF123:1?D6:<AFFFD94<<<?<24<:?22684)09F-4)0:<FFFA
@HWI-M00185:93:000000000-ABAB4:1:1101:14064:1617 2:N:0:GATCTG
CCCCGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCGGCGGCACAGAGGTCATGACAACCCCTACACCTAGTATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAAAGGCGCCTTCGCCACTGGTATTCTTCCTAATCCCTACGCATTTCACCGCTACCCTAGGAATTCACCTTTCCTTACCTGCCCTCTACATATCCAGTTTGGAATTCAGCA
+
-CCCCFEEGGGEDGGGGGGCFGGGCFFGGCFFFGGGGFEGGGGGGGGCFGGGGGGGFGGGGGGFGG@EGGGGGGGGGGGGGGGGGGGGFGFFGGGFGGGGGGG9BF9@FGGCFGFGGGGGDFGDGGGGGGGCDG,@BC:CEBFGGGCGGGCCFFGGGGGGD>D>EFFEE6FFGGGFFFCCECGGFFEF9<:FF85<7)7888(5E?FD:EFF)2<<99ABF:7<2:?AB#######################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:15060:1626 2:N:0:GATCTG
CCCCGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCGGCGGCACAGAGGTCATGACAACCCCTACACCTAGTATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAAAGGCGCCTTCGCCACTGGTATTCTTCCTAATCTCTACGCATTTCACCGCTACCCTAGGAATTCTCCTTTCCTCTCCTGCACTCTAGATATCCCGTTTGGAATGCAGCA
+
CCCCCGGGGGGGGGGGGGGFGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGEEGGGEFEGFGGGGG@,BEFGFGGGGGGGCCFGFCFFGGGDDFGGGEDCGFGGCDB8FC7;CGG?FGGE<C<?C55DG>;<(*3;D?CCAC);<C<AABFF*-).<BFFGADAA?>029((1(4CF4:24)5AFGF?BFF<(4))((.-54>>BF9A?2)(97FB##########
@HWI-M00185:93:000000000-ABAB4:1:1101:9832:1639 2:N:0:GATCTG
CCCCGTCAATTCATTTGAGTTTTAACCTTGCGACCGTACTCCCCAGGCGGTAAACTTAACGCGTTAGCTTCGGAGCCGAAGCCCAATCGCACAGAACCCCTGTTGATCACGTTTAAGGCGTGGAACAACAGGGTAATCTAACCTGTTTGCCTCCCACGGCTTTCAACCCTAGGGGTCATCTCTATACCGGGAGGCGCCTTCCGCCCCGGTATTCCTTCCTATCCCCTAGGCTTTCACCGCCAACCCTAGAATTTTAACCTCCTCCACCAGACCCTCGTAAACCCACTTCGAACACAGTCA
+
BCCCCFFGGGGG9FFFEGGFEFFGG@FGGFGG7FEGEGGGGGGGGGGGGG:6,:FFGG,BFEFEGGG<,CBCB+C?+F+@###########################################################################################################################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:23613:1647 2:N:0:GATCTG
CCCCGCCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCGGAGCCCACGCTCAATGGCACAGACTCCAAGTTGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCCGTATGCCCTCAGAACTCTCCCCATTTCACACCGCCACACGGAATTTTACCCCCCTCTACACGCCTCTAGTGCAACATCTTCACACCCATCTC
+
CCCCCEGGGGGGGGGGGGFFGG9F8<FFGGFFEEEEGGGGGGGGGGGGFF67BFGGGGAEFFEGGGEFFGGDGG:EB<EF@F,?==,<E,<FEGG8,A8F,9CAA,:>7A,B=EG:FE?@CF>9<EGC,+>A=DFDFG=E:>D=BGDAC7?=F:C7++31@+AEGGC,@0>;9A?##########################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:20000:1651 2:N:0:GATCTG
CCCCGTCAATTCATTTAAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTATCGCGTTAGCTTCGGAACCCACGCTCATAATGGCACAAACTCCAAATCGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACAAGCCTCTAGTCCGACCGTTTCAAATGCAGT
+
ACCCC@FFGGGGGGGGGGGFGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGG@FFGGGDGGGGGGGGGGGGGGGGGGGGG@FGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGFGGGEGGGFGGGFGGGGGFGGGGGGGGGGGGGGEGGEGEEGFGG6FED>4721:E>;A?EGGGGDGGGGGE@FFFEFFGGGD@>DDFFFF?(9?BB)6:AG?>AAD730201452:6592:<26@FFFBB9825-4))(,4245964))46(/7?.:7:D:AFF64
@HWI-M00185:93:000000000-ABAB4:1:1101:10499:1656 2:N:0:GATCTG
CCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCGGCAGCCACGTCTCAAGGACACAGCCTCCAAATCGACATCGTTTACGGCGTGGACTACCAGGGTATCTCATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCCGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACAAGACTCTCGCAGGCCAGTTTTAACTGCAATT
+
CCCCCGGGGGGGGGGGGGGGGGGGGGGGGFGGFGGGGGGGGGGGGGGGGCFECFEGFGFGGEGGGGEFFEFGG+@CFFFGDDFDEGGGDGGGGGGGGGGGGGF<E@AE+>FDFFGGAFFGEGGGGGGGGGDFGGGGGFG,EFGGGGFGG?CC8CFFGED>7CEGEEGC?C4E4<DF4)8?C=FGDF?E+>E7EDD):<@BFFCC(7A85BE########################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:9825:1657 2:N:0:GATCTG
CCCCGTCAATTCCTTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCGGAGCCCACGCTCAATGGCACAGACTCCAAGTTGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTCTGTCCCGGGGGCCGCCTTCGCCACCGGTATTCCCCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACAAGACTCTAATCTGACAGCTTCAAATGCAGTTC
+
8CCCCGEGGGGGGGGGGGGFGFGGGGGGGGGFGGGGGGGGGGGGGEGFGGGGGGGGGFGGGGGGGGGFFGGGGGGGGGGGGGGFGGGGGG8FG8FGEFGGFGGGGGGGGGGGGGGGG@FEFGGGGGGGGGGGDGG8EFGGGGGGGGGGFFD6A+C<FCCDD7CGGDGGFF5?FEGFFFFG9EFGGGC,=DDGCC9DDGDB?F0D*-51:CE35:)).(2810<-6469<C47?26).(4-,4<?<7029?466)56>@F:@08:A<A################################
@HWI-M00185:93:000000000-ABAB4:1:1101:20014:1662 2:N:0:GATCTG
CCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGACGACTTATCGCGTTAGCTTCGGCACCCACGCCCATAATGCCCCAACCCCCACATTGACACCTTTTCCAGCGTGGACTACCGGGTTATCTAATCCTTTTTTCTCCCCCCCCTTTCGCCCCTGAGCGTCGTTCTTTTCCCAGGGGCCCGCCTTCGCCACCGGTATTCCCCCAGATCTCAACTCACTCCACCACCCCACGTGAAATTCATCCCCCCTCTCCACGACTCTAGTCTGACCTCTCCAACTGACAT
+
CCCCC@FEGGFGFGGG,EFFGGGFGFGGFFFG7EGEGGGGGGGGGGGFC:+7+7@BCF,6CCFGGGDG,9C>E7FG7=F:FC++?,A,9A<B,@:F77@EFC+88,9,7,+,:5C,A,++6++3,6>+@++380=7@,@@;C,EA,;2BE@51**1/A6B*::A###################################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:20140:1666 2:N:0:GATCTG
CCCCGTCAATTCATTTAAGTTTTAATCTTGCGACCGTACTTCCCAGGCGGAATGCTTATTGCGTTAGCTGCGACACCGAAGGTGAAACCCCCAGCGTCTAGCATTCATCGTTTAGGGCATGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTTAGCGTCAGTTGAGACCCAGATAGCCGCCTTCGCCACTGGTGTTCCCCCTACTATCTACGAATTTCACTTCTACACTAGGAATTCCACTATCCTCTAACTCACACTAGCTTTAAAGTATTAAGTACCTTTCC
+
@CCCC,6@CGCC@<<EFFFFF@@FGGGGCEF:CCCGEFGGGGEFG8CFC@@FGFFFE@A<,CFE@CF<ACF7FGGGGEGEC7F<,:?FGGEF7CFGBF7=FCEA,EDFGGFECF<<4AFGGGEDFFD>D=F+:,<=@99,AEGGDDDF9DAFFDGCDGGF+@>D:E78CFFCD:C?9B=C7@9;4,=FFFDC<95=BFFGG265+0)/1)5:;?0=@+73591;/0/3;5<C3AAAA:):F:?:CFFAB#############################################
@HWI-M00185:93:000000000-ABAB4:1:1101:15007:1668 2:N:0:GATCTG
TCCCGTCAATTCCTTTGAGGTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGTTTTATCGCGTTAGCTTCGGAACCCACCCTCCTAGTGGCCCAGACTCCAAATCGACATCGTTTACAGCGTGGACTCCAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGCTATTCCTCCAGCTCTCTACGCATTTCACCGCCTCACCTGGAATTCTACCCCCCTCTACAAGACTCTAGTTACACAGTTTCAAATGCCGT
+
@CCCC,86<CF,<CF<,CEF@A@CC96E@CE:7CC@AEFGE<@DGG7C@C+,:@,CF,CF:>F,99,C7?:FF@FF,4,?,:5A5,8,>CE=DGGDE,<E7,8+6CB4,?FCF8F+8>>C8E,D::,++7@:DGD,3,73,3,C,=+<,@B:CDGCC7,4@C>E5CF,CB59)@@4@E+4>=;BFGE5?)77C5<24FGFBGGE##########################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:10515:1673 2:N:0:GATCTG
CCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCGGAAGCCACGTCCCAAGGACACAGCCTCCAAATCGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGGTTGCCCCCCACGCCTTTGCACCTGAGCGTCAGTTTTTGTCCCGGGGGCCGGCTTCGCCACCTGTATTCCTCCATATTGTCACCCATTTCAACCCTACACCTGGAATTCCAACCCCCTATACAAGACCTTCGCTGACCATTTTTACAACCAATT
+
CCCCCGGGGGGGGGGFGGGGGGCF@-FGGGGG+:FGGGGGGGGGGGGGGD@F@F,CFFF,FFGGGGGGGFGGGGG@GCGFC9=,8?AFGGCFGGFFAF=FGCGFEC:B:4A+:FG4E7FGECC7>,>8>FGCDC<83<=EFC,@;,@D3,2+<CGGGGG*;:,5DCF###################################################################################################################################
@HWI-M00185:93:000000000-ABAB4:1:1101:10167:1692 2:N:0:GATCTG
CCCCGCCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGAATACTTAATGCGTTAGCGGCGGCGCAGAGGTCATGACAACCCCTACACCTAGTATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAAAGTCGCCTTCGCCACTGGTATTCTTCCTAATCTCTACGCATTTCAACGCTACGCTAGGAATTCTACTTTCCTCTCCTGCACTCTAGATATCCAGTTTGGAATGCAGCA
+
ACCCCGGGGGGGGGGGGGGFGGGGGGGGFGGGDGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGEGGGGGGGGGGGGGGGGGFGGGGGGGGEGGGFGGGGGGFGGGGGGGFGGGGGGDGEFGGGGGGGGFGEFECEFCFGA;DGGGGGGGGGCDCFGGGGGGGGGFCGECFFGEFFFFCCFFGGGGGGGDGGGGGGC398;EFGGFD6<>7@5>:AF<AFB794542).92533CA<)66;22((,)495AF@BF=7F6>BF<8?FF?FB2594<)-644A<FFFFFG########

[westcott]

The barcode line is causing the error. It will be fixed in our next release.