Dist.seqs failed, is there a way to predict the size of the .dist file?

After running dist.seqs for over 3 days it finally failed, this was half expected as I don’t have too much space on my hard drive but I wanted to know if there is a way to predict the size of the .dist file for future reference and to avoid running the command pointlessly. Or am I better of just sticking to cluster.split? If so does this also require much space?

Sequence Time Num_Dists_Below_Cutoff
0 0 0
353300 336936 180284370
Killed: 9


Saving session…

…copying shared history…

…saving history…truncating history files…


Deleting expired sessions…none found.

[Process completed]

Thanks in advance.


Sorry you’re running into problems. cluster.split is probably your best bet.

Regardless, it looks like you have a lot of unique sequences. Are you sequencing the V4 region with 2x250 nt reads? If not, you’ll want to consult this…


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