Apologies in advance as I am very new to Mothur. I completed the classify otu command and I made a design file with my groups and treatments (made in excel, saved as tab-delimited). I am trying to run the get.groups command before I proceed with alpha/beta diversity, pcoa, and nmds, but I keep on getting these error messages:
“Unable to open final.design” “You must provide a design file when using the sets parameter”. The design file is saved in the correct working directory.
Any help is greatly appreciated.