Dear Mothur users
I hope everyone had a great week. I’m posting a query that has been posted before, but I couldn’t find a clear solution to this issue. I finished my workflow for another ITS dataset and got a .shared file at the end which looks like what I would expect from this system. However, when matching the otu data with my metadata, I realized that not all the samples downloaded into my folder from Basespace. More specifically, I was missing three samples (out of 300). I wasn’t aware of the fact that I had to run pre.cluster on all my samples together, so I just ran these three samples separately on my workflow and got a separate .shared file. I just realized that you can’t simply combine the two .shared excel files. As far as I understand, I need to merge the two fasta files and group files (latest version before pre.cluster) and then run pre.cluster on the combined files. I do not know how to merge these two group files. If there is an easy way to address this issue that someone is aware of, I would dearly appreciate your help.