Hello community, Pat
I have a question regarding the clustering steps to generate OTUs. I am analyzing a 18S-V4, MiSeq data set.
I used cluster.split as recommended by the MiSeq SOP, but then wanted to use get.oturep, command that requires a .dist file.
Should I just run dist.seqs()? If so, any caveats about the file inputs I should be aware of?
Thanks for the help, I am new and get easily confused when making decisions on what is accurate.