archive of mock community taxonomy results

Feature requests
1

Hi Pat and mothur Community,
I was wondering if there is any interest to create some kind of repository where people can upload taxonomy results obtained from sequencing mock bacterial communities. We routinely include the 20-species Microbial Mock Community B (BEI Resources cat no. HM-782D). Using V1-V2 primers and MiSeq 300-nt single-end sequencing, we consistently obtain ~ 1/2 the number of Proteobacteria reads and ~ 3 time more Baceroidetes that expected from the product description. I would be curious to see whether other folks using this or similar controls obtain similar results, whether this is related to the primers or something else.

thanks,

Giovanni

2

I’d be thrilled if there was a way to represent these results some how. In the 454 SOP paper we found similar things for the Proteos with the V13 region because the 27f/8f primer had mismatches with E. coli (and Bifido).

3

so how do we go about posting such data? I would be happy to provide classify.seqs results - or similar data - obtained from bacterial DNA controls.

Giovanni