hello, I am interested in working with archaea, my primers have coverage for both bacteria and archaea. To get more archaeal sequences, is it necessary to modify a parameter of the MiSeq SOP protocol?
Best regards!
Hi Diego,
You’d just want to make sure that the classification database files you use have good representation of archaea. I don’t do much with Archaea so I can’t give you a good assessment of which dataset works best. I suspect the SILVA reference is what you want to use
Pat