AMOVA error

Commands in mothur
1

When I run amova command, it gave the hint:
Experiment-wise error rate: 0.05
Pair-wise error rate (Bonferroni): 0.0166667
If you have borderline P-values, you should try increasing the number of iterations

What the exact meaning for these? And I also found the p-value was not the same when I executed the command again.

Thank you for your time!

Best!

2

It’s a permutation-based test so the pvalues will vary slightly between runs. Your pvalues should be below 0.017 to be considered significant.