Alpha and beta diversity

Dear All,

I have sequencing data from three compost sources and would like to compare their alpha and beta diversity to determine whether any source exhibits greater microbial diversity. I attempted to perform the analysis in mothur using commands from the SOP, but the workflow did not run successfully. Has anyone used mothur for diversity analysis and, if so, would you be willing to share the commands or workflow you used? Thank you!

Hi there - we follow those commands in the SOP in my lab. What are you having problems with? If you can post the first error message you’re running into along with the syntax of your command we can help you out.

Pat