Hello,
I am in the process of analyzing some MiSeq 250PE data (16S, 515F/806R). I have been been through the SOP and I am now running one of my samples. At the mothur > screen.seqs step, I have gotten an error regarding missing sequences (pasted below). I am not sure how to deal with this error.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_24648_16337 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_25527_24646 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_26180_18930 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_26589_23793 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_27283_14101 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_27526_15744 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_28064_13998 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_4901_12572 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_5979_17408 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_7227_20645 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_7691_12299 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_7710_12305 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_7848_13677 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_8131_21493 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_8480_5582 please correct.
Your count file does not include the sequence HWI-M01141_82_A56CJ_1_2114_9379_11699 please correct.
Any comments would be greatly appreciated.
thanks,
d