Trouble shoting of cluster

Commands in mothur
21

Dear Dr. Pschloss,

I tried
mothur > screen.seqs(fasta=./align/454.trim.cut.unique.align,name=./unique/454.trim.cut.names,group=./trim/454.groups,minlength=200,maxambig=0,maxhomop=8,processors=10, outputdir=./screen/, start=10352)

and

mothur > summary.seqs(fasta=./filter/454.trim.cut.unique.good.filter.fasta, name=./screen/454.trim.cut.good.names, processors=10)

and got this result


Start End NBases Ambigs Polymer NumSeqs Minimum: 1 19 7 0 2 1 2.5%-tile: 1 39 17 0 3 14271 25%-tile: 1 39 17 0 3 142704 Median: 1 39 17 0 3 285407 75%-tile: 1 39 17 0 3 428110 97.5%-tile: 1 39 17 0 3 556543 Maximum: 3 243 218 0 8 570813 Mean: 1.00009 39.2428 16.9865 0 2.99259 # of unique seqs: 378987 total # of seqs: 570813

please help me

22

What is the output of summary.seqs on the output of screen.seqs? what you sent was the output from after running filter.seqs.