Get.otus results in error

Hi,

I have been trying to follow Pats advice to convert mothur output to allow its use in picrust. Link here: Mothur file to biom

When I do the get.otus(constaxonomy= stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.opti_mcc.0.03.cons.taxonomy, accnos= stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.opti_mcc.list) step I get the following error.

[ERROR]: 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used: 0.017601Gigabytes . Total Ram: 125.364Gigabytes.

has occurred in the Utils class function mothurConvert-int. Please contact Pat Schloss at mothur.bugs@gmail.com, and be sure to include the mothur.logFile with your inquiry.

Could you please advise on how to proceed?
Thanks
Paul

The issue is the accnos file. It looks like you provided a list file instead of a file containing the OTU labels.

get.otus(constaxonomy= stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.opti_mcc.0.03.cons.taxonomy, accnos= stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.opti_mcc.list)

The list.otus command will generate the accnos file for you. Try this:

list.otus(list=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.opti_mcc.list)
get.otus(accnos=current, constaxonomy= stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.opti_mcc.0.03.cons.taxonomy)