I must need your help.
I am running mothur 1.41 in biolinux machine. I am analysing 16S rRNA illumina sequencing data. All my data is in an external hard-drive connected to the machine via usb port. Mothur is running directly in the data folder, so in the external hard-drive (4 Tb space). I decided to run all in the external drive due to a previous killed9 error, guessing a rather disk space issue than a software issue. Analyseswent smooth until analysing sequences at OTU level.
Dist.seqs takes its time, but finally complete .
However, while running cluster.split … it crashed with the message “zsh killed”.
Do you have any idea? can not be a disk space issue, there are still 2Tb free space. So I am wondeirng if it’s just a problem due to running mothur in an external drive or … buffering problem. In fact, I have no clues … any idea from the audience?
Thank you very much for any help of suggestion.