# Your count file does not include the sequence...

**URL:** <https://forum.mothur.org/t/your-count-file-does-not-include-the-sequence/1498>\
**Category:** Journal club\
**Created:** [September 11, 2013, 2:22pm UTC](https://forum.mothur.org/t/your-count-file-does-not-include-the-sequence/1498 "2013-09-11T14:22:54Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![dlearman](https://avatars.discourse-cdn.com/v4/letter/d/4da419/32.png) [@dlearman](https://forum.mothur.org/u/dlearman)\
**Post date:** [September 11, 2013, 2:22pm UTC](https://forum.mothur.org/t/your-count-file-does-not-include-the-sequence/1498/1 "2013-09-11T14:22:54Z")

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Hello,

I am in the process of analyzing some MiSeq 250PE data (16S, 515F/806R). I have been been through the SOP and I am now running one of my samples. At the mothur \> screen.seqs step, I have gotten an error regarding missing sequences (pasted below). I am not sure how to deal with this error.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_24648\_16337 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_25527\_24646 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_26180\_18930 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_26589\_23793 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_27283\_14101 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_27526\_15744 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_28064\_13998 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_4901\_12572 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_5979\_17408 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_7227\_20645 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_7691\_12299 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_7710\_12305 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_7848\_13677 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_8131\_21493 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_8480\_5582 please correct.  
Your count file does not include the sequence HWI-M01141\_82\_A56CJ\_1\_2114\_9379\_11699 please correct.

Any comments would be greatly appreciated.

thanks,  
d