# XXX is not in your name or count file, please correct **URL:** \ **Category:** Commands in mothur\ **Created:** [September 22, 2022, 5:30pm UTC](https://forum.mothur.org/t/xxx-is-not-in-your-name-or-count-file-please-correct/21561 "2022-09-22T17:30:49Z")\ **Posts on this page:** 4\ **Page:** 1
**Author:** ![sokratis](https://avatars.discourse-cdn.com/v4/letter/s/85f322/32.png) [@sokratis](https://forum.mothur.org/u/sokratis)\ **Post date:** [September 22, 2022, 5:30pm UTC](https://forum.mothur.org/t/xxx-is-not-in-your-name-or-count-file-please-correct/21561/1 "2022-09-22T17:30:49Z")
Hi there, Not an expert, new to the program… I have run several examples following the MiSOP command sequence and things have going smoothly but when I try to run my real samples things start to go wrong after the align.seqs and screen.seqs… I have tried 3 times starting from scratch but no luck. I attach the part of the logfile. mothur \> align.seqs(fasta=stability.trim.contigs.good.unique.fasta, reference=silva.v4.fasta) Using 16 processors. Reading in the silva.v4.fasta template sequences… DONE. It took 3 to read 14956 sequences. Aligning sequences from stability.trim.contigs.good.unique.fasta … It took 1475 secs to align 4115140 sequences. [WARNING]: 5079 of your sequences generated alignments that eliminated too many bases, a list is provided in stability.trim.contigs.good.unique.flip.accnos. [NOTE]: 3759 of your sequences were reversed to produce a better alignment. It took 1485 seconds to align 4115140 sequences. Output File Names: stability.trim.contigs.good.unique.align stability.trim.contigs.good.unique.align\_report stability.trim.contigs.good.unique.flip.accnos > summary.seqs(fasta=stability.trim.contigs.good.unique.align, count=stability.trim.contigs.good.count\_table) Using 16 processors. ``` Start End NBases Ambigs Polymer NumSeqs ``` Minimum: 0 0 0 0 1 1 2.5%-tile: 1 18929 439 0 4 259538 25%-tile: 1 18929 440 0 5 2595376 Median: 1 18929 443 0 5 5190751 75%-tile: 1 18929 460 0 6 7786126 97.5%-tile: 2 18929 466 0 6 10121963 Maximum: 18929 18929 494 0 8 10381500 Mean: 6 18903 449 0 5 # of unique seqs: 4115140 total # of seqs: 10381500 It took 502 secs to summarize 10381500 sequences. Output File Names: stability.trim.contigs.good.unique.summary mothur \> screen.seqs(fasta=stability.trim.contigs.good.unique.align, count=stability.trim.contigs.good.count\_table, start=2, end=18929) Using 8 processors. 1000 1000 1000 … 257196 It took 176 secs to screen 4115140 sequences, removed 124339. /\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*/ Running command: remove.seqs(accnos=stability.trim.contigs.good.unique.bad.accnos.temp, count=stability.trim.contigs.good.count\_table) Removed 14 sequences from stability.trim.contigs.good.count\_table. Output File Names: stability.trim.contigs.good.pick.count\_table /\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*/ Output File Names: stability.trim.contigs.good.unique.good.align stability.trim.contigs.good.unique.bad.accnos stability.trim.contigs.good.good.count\_table It took 675 secs to screen 4115140 sequences. mothur \> summary.seqs(fasta=stability.trim.contigs.good.unique.align, count=stability.trim.contigs.good.good.count\_table) Using 16 processors. [ERROR]: ‘M07149\_18\_000000000-KGDT6\_1\_2101\_17402\_11050’ is not in your name or count file, please correct. [ERROR]: ‘M07149\_18\_000000000-KGDT6\_1\_2112\_10437\_9521’ is not in your name or count file, please correct. Any help greatly appreciated. ---
**Author:** ![sokratis](https://avatars.discourse-cdn.com/v4/letter/s/85f322/32.png) [@sokratis](https://forum.mothur.org/u/sokratis)\ **Post date:** [September 23, 2022, 11:41pm UTC](https://forum.mothur.org/t/xxx-is-not-in-your-name-or-count-file-please-correct/21561/2 "2022-09-23T23:41:23Z")
OK. After exploring various other issues, I figured out mine. Not enough disk space. Deleted files, increased size of partition and so far so good. Also based on the suggestion of a colleague I analysed up to align smaller batches of samples which i merged afterwards. Hope this is useful to others. Cheers. ---
**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\ **Post date:** [September 30, 2022, 12:47pm UTC](https://forum.mothur.org/t/xxx-is-not-in-your-name-or-count-file-please-correct/21561/3 "2022-09-30T12:47:39Z")
Glad you figured it out - the reason you are running out of space is, in part, because you sequenced a region where your reads do not fully overlap with each other. When reads do not fully overlap the error rate is about 10x that of when they do: > **[Why do I have such a large distance matrix](https://mothur.org/blog/2014/Why-such-a-large-distance-matrix/)** > > The website that supports the mothur software program - one of the most widely used tools for analyzing 16S rRNA gene sequence data. Step inside to learn how to use the software, get help, and join our community! ---
**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\ **Post date:** [October 10, 2022, 12:48pm UTC](https://forum.mothur.org/t/xxx-is-not-in-your-name-or-count-file-please-correct/21561/4 "2022-10-10T12:48:07Z")
This topic was automatically closed 10 days after the last reply. New replies are no longer allowed.