# \[WARNING\]: 182190 of your sequences generated alignments that eliminated too many bases,

**URL:** https://forum.mothur.org/t/warning-182190-of-your-sequences-generated-alignments-that-eliminated-too-many-bases/21521
**Category:** Commands in mothur
**Created:** [August 5, 2022, 8:22pm UTC](https://forum.mothur.org/t/warning-182190-of-your-sequences-generated-alignments-that-eliminated-too-many-bases/21521 "2022-08-05T20:22:36Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![SNOSO](https://avatars.discourse-cdn.com/v4/letter/s/e19adc/32.png) [@SNOSO](https://forum.mothur.org/u/SNOSO)
#### Post date: [August 5, 2022, 8:22pm UTC](https://forum.mothur.org/t/warning-182190-of-your-sequences-generated-alignments-that-eliminated-too-many-bases/21521/1 "2022-08-05T20:22:36Z")

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Hi there, im having trouble figuring out why so many sequences had bases eliminated. I have attached a google doc with the pipeline and the outputs from the corresponding line of input coding which can be found [here](https://docs.google.com/document/d/1EoT-Dv6RErdRckx05-K8f0kP1MsCW1rljWK1crgFK2s/edit?usp=sharing).

Any advice would be appreciated!

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### Author: ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)
#### Post date: [August 9, 2022, 3:07pm UTC](https://forum.mothur.org/t/warning-182190-of-your-sequences-generated-alignments-that-eliminated-too-many-bases/21521/2 "2022-08-09T15:07:09Z")

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Hi SNOSO,

I don’t have access to your google doc. Could you just post the output that mothur put to the screen?

Pat

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### Author: ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)
#### Post date: [August 19, 2022, 3:07pm UTC](https://forum.mothur.org/t/warning-182190-of-your-sequences-generated-alignments-that-eliminated-too-many-bases/21521/3 "2022-08-19T15:07:15Z")

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