# Using V19 RDP Reference File with Galaxy Tutorial **URL:** \ **Category:** Commands in mothur\ **Created:** [July 2, 2024, 3:15pm UTC](https://forum.mothur.org/t/using-v19-rdp-reference-file-with-galaxy-tutorial/22178 "2024-07-02T15:15:11Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![jmkagle](https://avatars.discourse-cdn.com/v4/letter/j/b9bd4f/32.png) [@jmkagle](https://forum.mothur.org/u/jmkagle)\ **Post date:** [July 2, 2024, 3:15pm UTC](https://forum.mothur.org/t/using-v19-rdp-reference-file-with-galaxy-tutorial/22178/1 "2024-07-02T15:15:11Z")
I’m fairly new to this, so I’m sorry if this is a newbie question. I tried to download and use the Version 19 16S rRNA Reference (PDS) instead of the older provided taxonomy files in the Galaxy 16S Microbial Analysis with mothur (extended) tutorial ([Hands-on: 16S Microbial Analysis with mothur (extended) / 16S Microbial Analysis with mothur (extended) / Microbiome](https://training.galaxyproject.org/training-material/topics/microbiome/tutorials/mothur-miseq-sop/tutorial.html)) and the Classify.seqs step failed. At first it wouldn’t run because the file type was wrong, but I fixed that (I think). Is there something else I need to do to get Classify.seqs to work with the new reference files in the tutorial? I got the following error for the failed taxonomy file: /corral4/main/jobs/058/867/58867357/tool\_script.sh: line 23: 11594 Done echo ‘classify.seqs( fasta=fasta.dat, reference=alignment.template.dat, taxonomy=tax.taxonomy.dat, method=wang, ksize=8, iters=100, cutoff=80, probs=true, count=count.dat, relabund=false, output=simple, printlevel=-1, processors=’${GALAXY\_SLOTS:-8}’ )’ 11595 | sed ‘s/ //g’ 11596 Segmentation fault (core dumped) | ./mothur 11597 | tee mothur.out.log ---
**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\ **Post date:** [July 8, 2024, 7:48pm UTC](https://forum.mothur.org/t/using-v19-rdp-reference-file-with-galaxy-tutorial/22178/2 "2024-07-08T19:48:10Z")
Hi there, It looks like the galaxy folks might be doing something to the files to generate that type of code. I’d suggest following up wiht them. I really don’t know much about how galaxy works. Sorry! Pat ---
**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\ **Post date:** [July 18, 2024, 7:48pm UTC](https://forum.mothur.org/t/using-v19-rdp-reference-file-with-galaxy-tutorial/22178/3 "2024-07-18T19:48:21Z")
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