# Using MED (minimum entropy decomposition)

**URL:** <https://forum.mothur.org/t/using-med-minimum-entropy-decomposition/3011>\
**Category:** Integrating mothur with other programs\
**Created:** [October 7, 2016, 9:49am UTC](https://forum.mothur.org/t/using-med-minimum-entropy-decomposition/3011 "2016-10-07T09:49:54Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![kristinvh](https://avatars.discourse-cdn.com/v4/letter/k/d07c76/32.png) [@kristinvh](https://forum.mothur.org/u/kristinvh)\
**Post date:** [October 7, 2016, 9:49am UTC](https://forum.mothur.org/t/using-med-minimum-entropy-decomposition/3011/1 "2016-10-07T09:49:54Z")

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Hi

I am using 16s data generated from the Miseq illumina SOP and I would like to try MED ([http://merenlab.org/2014/11/04/med/#the-manuscript](http://merenlab.org/2014/11/04/med/#the-manuscript)) on the quality filtered-sequences (not the unique sequences). Which of the files is most appropriate to use, this one: stability.trim.contigs good fasta (around 3 GB for my data). ?

Thank you!

Kristin