# Unclassified fungi (Fungal analysis ITS2 region)

**URL:** <https://forum.mothur.org/t/unclassified-fungi-fungal-analysis-its2-region/20544>\
**Category:** Theory behind mothur\
**Created:** [May 29, 2020, 3:12am UTC](https://forum.mothur.org/t/unclassified-fungi-fungal-analysis-its2-region/20544 "2020-05-29T03:12:09Z")\
**Posts on this page:** 1\
**Showing post:** 3

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**Author:** ![Faisal](https://avatars.discourse-cdn.com/v4/letter/f/fbc32d/32.png) [@Faisal](https://forum.mothur.org/u/Faisal)\
**Post date:** [June 3, 2020, 1:22pm UTC](https://forum.mothur.org/t/unclassified-fungi-fungal-analysis-its2-region/20544/3 "2020-06-03T13:22:38Z")

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Hi Kendra

Thanks for your reply.  
Yes I tried plating the rep sequences using the UNITE, ITS NCBI, and NCBI RefSeq databases. No hits or similarities were found! Very frustrating.

Thanks again  
Faisal

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