# Unable to open silva.seed\_v138.1

**URL:** https://forum.mothur.org/t/unable-to-open-silva-seed-v138-1/22130
**Category:** mothur bugs
**Created:** [April 16, 2024, 1:45am UTC](https://forum.mothur.org/t/unable-to-open-silva-seed-v138-1/22130 "2024-04-16T01:45:32Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![jenam](https://avatars.discourse-cdn.com/v4/letter/j/ac8455/32.png) [@jenam](https://forum.mothur.org/u/jenam)
#### Post date: [April 16, 2024, 1:45am UTC](https://forum.mothur.org/t/unable-to-open-silva-seed-v138-1/22130/1 "2024-04-16T01:45:32Z")

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Hello, from jenam who is a fresh man for bioinformatics 🙂

I have some troubles with my work

 ![mothur](https://canada1.discourse-cdn.com/flex036/uploads/mothur/original/1X/14e12fc2d699362e1768c621a7420ff4f2513850.gif)

I’d like to do sequence alignment for 16s rRNA gene sequences

I guess I have to set my directory for all analysis files, right?

Want to know what I have to do firstly 🙂

Plz help 🙂

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### Author: ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)
#### Post date: [April 16, 2024, 9:31pm UTC](https://forum.mothur.org/t/unable-to-open-silva-seed-v138-1/22130/2 "2024-04-16T21:31:00Z")

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Hi,

I’d encourage you to put all of your data files, reference files, and mothur program in a single directory - there should be only files in that directory, no directories. Then try double clicking on the mothur executable and run the commands.

Pat

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### Author: ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)
#### Post date: [April 26, 2024, 9:31pm UTC](https://forum.mothur.org/t/unable-to-open-silva-seed-v138-1/22130/3 "2024-04-26T21:31:59Z")

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