# trim.seqs-problem with qfile
**URL:** https://forum.mothur.org/t/trim-seqs-problem-with-qfile/3056
**Category:** Commands in mothur
**Created:** [December 5, 2016, 12:17pm UTC](https://forum.mothur.org/t/trim-seqs-problem-with-qfile/3056 "2016-12-05T12:17:34Z")
**Posts on this page:** 3
**Page:** 1
### Author:  [@Helen](https://forum.mothur.org/u/Helen)
#### Post date: [December 5, 2016, 12:17pm UTC](https://forum.mothur.org/t/trim-seqs-problem-with-qfile/3056/1 "2016-12-05T12:17:34Z")
Hello,
I have data from 454 pyrosequencing. I used for denoising my data: mothur \> shhh.flows(flow=sample.flow, order=B, LookUp\_Titanium.pat). Outputs were used in next step with the command “trim.seqs” and parameter “qaverage”: mothur \> trim.seqs(fasta=sample.shhh.fasta, qfile=sample.shhh.qual, qaverage=25). Program wrote “Error in reading your qfile, at position -1. Blank name” and mothur falled down. Could somebody help me with this problem? Many thanks.
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### Author:  [@pschloss](https://forum.mothur.org/u/pschloss)
#### Post date: [December 5, 2016, 1:50pm UTC](https://forum.mothur.org/t/trim-seqs-problem-with-qfile/3056/2 "2016-12-05T13:50:33Z")
If you’re doing shhh.flows, you don’t need to run trim.seqs with quality score values. The shhh.flows qscore values are pretty meaningless. Checkout the pipeline at [https://mothur.org/wiki/454\_SOP](https://mothur.org/wiki/454_SOP)
Pat
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### Author:  [@Helen](https://forum.mothur.org/u/Helen)
#### Post date: [December 6, 2016, 7:38am UTC](https://forum.mothur.org/t/trim-seqs-problem-with-qfile/3056/3 "2016-12-06T07:38:20Z")
Many thanks.
Helen