mothur

Summary.seqs error after make.contigs

Hi all,

I am getting the following error message when I try to execute summary.seqs on the Galaxy database (below). I would greatly appreciate any suggestions on how I can fix this. Thanks!

[He[J

mothur > summary.seqs(fasta=fasta.dat,processors=6)
[ERROR]: fasta.dat is blank, aborting.
You have no current fastafile and the fasta parameter is required.

Using 6 processors.
[ERROR]: did not complete summary.seqs.

mothur > quit




Detected 2 [ERROR] messages, please review.




Sorry, but we have nothing to do with the galaxy implementation of mothur. I know that they’re often a few versions behind the current version of mothur.

That error message means that there’s either nothing in the file or the file doesn’t exist.