# Subsampled Data

**URL:** <https://forum.mothur.org/t/subsampled-data/2071>\
**Category:** Commands in mothur\
**Created:** [October 13, 2014, 5:51pm UTC](https://forum.mothur.org/t/subsampled-data/2071 "2014-10-13T17:51:32Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![remillard](https://avatars.discourse-cdn.com/v4/letter/r/73ab20/32.png) [@remillard](https://forum.mothur.org/u/remillard)\
**Post date:** [October 13, 2014, 5:51pm UTC](https://forum.mothur.org/t/subsampled-data/2071/1 "2014-10-13T17:51:32Z")

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HI All,

I have completed the analysis of miseq data using mother (_cheer_). I have my subsampled file giving me reads per OTU:

sampleX.trim.contigs.good.unique.good.filter.unique.precluster.pick.pds.wang.pick.tx.1.subsample.shared

How do I turn the OTUs used in this file in to taxonomic labels (yes I am a taxonomy addict).

Thanks for any help much appreciated.

Rem

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [October 14, 2014, 6:55pm UTC](https://forum.mothur.org/t/subsampled-data/2071/2 "2014-10-14T18:55:20Z")

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You would have to subsample the list file. You could do the following:

sub.sample(list=sampleX.trim.contigs.good.unique.good.filter.unique.precluster.pick.pds.wang.pick.tx.list, name=yourNameFile, taxonomy=yourTaxonomyFile, name=yourNameFile, group=yourGroupFile, persample=yourChoice) - subsample all files  
make.shared(list=current, group=current) - created shared file from subsampled files  
classify.otu(list=current, taxonomy=current, name=current, group=current) - classify subsampled otus