stability.trim.contigs.good.unique.good.align is empty!

mothur bugs
1

Hi everyone,

I’m trying to run mothur using two 16S samples from an specific organism. Well, I started with the MiSeq SOP reference files (RDP taxonomic set and Silva database), but in order to become more accurate, I downloaded the new releases from those databanks.

So, the problem is when I do the align.seqs command and the output is a stability.trim.contigs.good.unique.good.align empty… I tried to search for an answer, but I also thought that is more efficient to ask it for you guys.

And I’m using the Silva.seed v123 release…

Thanks,
Rafael.

2

Ok, I solved the problem…

It was due to the similar name of the reference database… I just changed to another name and apparently it’s working.
I could delete the post, but I don’t know if any other user can have the same problem…

Thanks,
Rafael.