# Query regarding otu file generation

**URL:** <https://forum.mothur.org/t/query-regarding-otu-file-generation/21627>\
**Category:** Commands in mothur\
**Created:** [November 15, 2022, 7:41pm UTC](https://forum.mothur.org/t/query-regarding-otu-file-generation/21627 "2022-11-15T19:41:51Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![abhisek001](https://avatars.discourse-cdn.com/v4/letter/a/ecb155/32.png) [@abhisek001](https://forum.mothur.org/u/abhisek001)\
**Post date:** [November 15, 2022, 7:41pm UTC](https://forum.mothur.org/t/query-regarding-otu-file-generation/21627/1 "2022-11-15T19:41:51Z")

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Hello there. Please let me know which command will generate OTU table?

Is the following an example of an OTU file? but I need OTU table.  
final.opti\_mcc.list

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<div class="post-metadata">

**Author:** ![leocadio](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/leocadio/32/203_2.png) [@leocadio](https://forum.mothur.org/u/leocadio)\
**Post date:** [November 16, 2022, 2:13pm UTC](https://forum.mothur.org/t/query-regarding-otu-file-generation/21627/2 "2022-11-16T14:13:36Z")

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maybe [get.oturep](https://mothur.org/wiki/get.oturep/) but I am not sure at which point you are, to be able to help you

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**Author:** ![abhisek001](https://avatars.discourse-cdn.com/v4/letter/a/ecb155/32.png) [@abhisek001](https://forum.mothur.org/u/abhisek001)\
**Post date:** [November 16, 2022, 5:42pm UTC](https://forum.mothur.org/t/query-regarding-otu-file-generation/21627/3 "2022-11-16T17:42:45Z")

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Thanks for your response actully It is taking a very much long time to run dist.seq command can I get help regarding this command how can I get meaningful output with in minimal time with this command. How much memory space we should have for 12 forward and reverse sample I have V3V4 16S amplicon sequencing. Thank you for listening to me.

```auto
dist.seqs(fasta=amazon.unique.filter.fasta, cutoff=0.10)

```

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<div class="post-metadata">

**Author:** ![leocadio](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/leocadio/32/203_2.png) [@leocadio](https://forum.mothur.org/u/leocadio)\
**Post date:** [November 16, 2022, 8:44pm UTC](https://forum.mothur.org/t/query-regarding-otu-file-generation/21627/4 "2022-11-16T20:44:18Z")

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Not sure of the dist.seqs. Have you run pre.cluster?

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<div class="post-metadata">

**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\
**Post date:** [November 26, 2022, 8:44pm UTC](https://forum.mothur.org/t/query-regarding-otu-file-generation/21627/5 "2022-11-26T20:44:33Z")

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