# Problems with summary.seqs
**URL:** https://forum.mothur.org/t/problems-with-summary-seqs/20428
**Category:** Commands in mothur
**Created:** [April 3, 2020, 12:28am UTC](https://forum.mothur.org/t/problems-with-summary-seqs/20428 "2020-04-03T00:28:32Z")
**Posts on this page:** 5
**Page:** 1
### Author:  [@Equiroz](https://forum.mothur.org/u/Equiroz)
#### Post date: [April 3, 2020, 12:28am UTC](https://forum.mothur.org/t/problems-with-summary-seqs/20428/1 "2020-04-03T00:28:32Z")
Hello, how are you? I have a problem during an analysis of phage therapy samples, when I get to the summary.seqs section, it indicates the following error:
mothur \> summary.seqs(fasta=current, count=current)
Using /home/equiroz/Artemia/stability.trim.contigs.good.good.count\_table as input file for the count parameter.
Using /home/equiroz/Artemia/stability.trim.contigs.good.unique.good.align as input file for the fasta parameter.
Using 64 processors.
[ERROR]: /home/equiroz/Artemia/stability.trim.contigs.good.unique.good.align is blank. Please correct.
Error in reading your fastafile, at position -1. Blank name.
Could someone give me some advice on this
Kind regards
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### Author:  [@pschloss](https://forum.mothur.org/u/pschloss)
#### Post date: [April 6, 2020, 6:41pm UTC](https://forum.mothur.org/t/problems-with-summary-seqs/20428/2 "2020-04-06T18:41:17Z")
It looks like you ran `screen.seqs` before running `align.seqs`. Can you send us the command syntax you used on `screen.seqs`? Also, it would be great if you could include the output from running `summary.seqs` on `stability.trim.contigs.good.unique.align` and `stability.trim.contigs.good.unique.good.align`. I think the second `*.good.align` will be empty.
Pat
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### Author:  [@Equiroz](https://forum.mothur.org/u/Equiroz)
#### Post date: [April 9, 2020, 8:09pm UTC](https://forum.mothur.org/t/problems-with-summary-seqs/20428/3 "2020-04-09T20:09:15Z")
Thank you very much for the information, I attach the command syntax that it used until reaching screen.seqs and the results you requested, because now I cannot advance in this step summary.single **(shared = stability.opti\_mcc.shared, cal = nseqs -coverage-sobs-npshannon-chao, subsample = XXX)**, thank you very much for the help, I will be waiting for your observations to be able to advance
mothur \> make.file(inputdir=file, type=fastq, prefix=stability)
mothur \> make.contigs(file=stability.files, processors=16)
mothur \> summary.seqs(fasta=stability.trim.contigs.fasta)
mothur \> screen.seqs(fasta=stability.trim.contigs.fasta,group=stability.contig.groups, maxambig=0, maxlength=275)
mothur \> get.current()
mothur \> summary.seqs(fasta=stability.trim.contigs.good.fasta)
mothur \> summary.seqs(fasta=current)
mothur \> summary.seqs()
mothur \> unique.seqs(fasta=stability.trim.contigs.good.fasta)
mothur \> count.seqs(name=stability.trim.contigs.good.names, group=stability.contigs.good.groups)
mothur \> summary.seqs(count=stability.trim.contigs.good.count\_table)
mothur \> align.seqs(fasta=stability.trim.contigs.good.unique.fasta, reference=silva.bacteria.fasta)
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### Author:  [@pschloss](https://forum.mothur.org/u/pschloss)
#### Post date: [April 16, 2020, 4:24pm UTC](https://forum.mothur.org/t/problems-with-summary-seqs/20428/4 "2020-04-16T16:24:05Z")
How about the output to the screen from running `summary.seqs` at the various stages?
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### Author:  [@system](https://forum.mothur.org/u/system)
#### Post date: [April 26, 2020, 4:36pm UTC](https://forum.mothur.org/t/problems-with-summary-seqs/20428/5 "2020-04-26T16:36:23Z")
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