# Problems with make.sra in v1.36.1

**URL:** <https://forum.mothur.org/t/problems-with-make-sra-in-v1-36-1/2740>\
**Category:** mothur bugs\
**Created:** [March 3, 2016, 5:12pm UTC](https://forum.mothur.org/t/problems-with-make-sra-in-v1-36-1/2740 "2016-03-03T17:12:11Z")\
**Posts on this page:** 2\
**Page:** 1

<div class="post-metadata">

**Author:** ![caiorachid](https://avatars.discourse-cdn.com/v4/letter/c/a5b964/32.png) [@caiorachid](https://forum.mothur.org/u/caiorachid)\
**Post date:** [March 3, 2016, 5:12pm UTC](https://forum.mothur.org/t/problems-with-make-sra-in-v1-36-1/2740/1 "2016-03-03T17:12:11Z")

</div>

I have tried to run make.sra in mothur v 1.36.1 and I got this:

mothur \> make.sra(fastq=siqoriginal.fastq, oligos=caries.oligos, project=project.txt, mimark=caries.tsv, platform=ILLUMINA, libstrategy=amplicon, instrument=Illumina\_MiSeq)

/\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*/  
Running command: fastq.info(fasta=f, qfile=f, fastq=siqoriginal.fastq, oligos=caries.oligos)  
[ERROR]: no outputs selected. Aborting.  
/\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*/

Output File Names:  
submission.xml

The problem seems to be in the fastq.info with both fasta and qfile in “F”.  
The command fastq.info alone works without this options.

I used then the v 1.35 to run make.sra and it worked just fine.

Thanks!

---

<div class="post-metadata">

**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [March 8, 2016, 1:14pm UTC](https://forum.mothur.org/t/problems-with-make-sra-in-v1-36-1/2740/2 "2016-03-08T13:14:23Z")

</div>

Thanks for reporting this bug. It effects the make.sra command with a single fastq file and oligos file and will be corrected in our next release coming soon. I am glad you were able to work around it with version 1.35.
