# Problem cluster.split

**URL:** <https://forum.mothur.org/t/problem-cluster-split/21760>\
**Category:** Commands in mothur\
**Created:** [March 20, 2023, 10:22pm UTC](https://forum.mothur.org/t/problem-cluster-split/21760 "2023-03-20T22:22:12Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![PeterJS0126](https://avatars.discourse-cdn.com/v4/letter/p/b4bc9f/32.png) [@PeterJS0126](https://forum.mothur.org/u/PeterJS0126)\
**Post date:** [March 20, 2023, 10:22pm UTC](https://forum.mothur.org/t/problem-cluster-split/21760/1 "2023-03-20T22:22:12Z")

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Hi guys, I have a problem with running cluster.split of mothur. I used “cluster.split(fasta=ok.abund.fasta, count=ok.abund.count\_table, taxonomy=ok.taxonomy, taxlevel=4, cutoff=0.03, processors=10)” and give this error:

Selecting sequences for group Gammaproteobacteria\_unclassified (12 of 101)  
Number of unique sequences: 10484

[WARNING]: ok.abund.count\_table does not contain any sequence from the .accnos file.  
Selected 0 sequences from ok.abund.count\_table.

Calculating distances for group Gammaproteobacteria\_unclassified (12 of 101):  
[ERROR]: you must have at least 2 sequences to calculate the distances, aborting.  
Segmentation fault (core dumped)

Also, I wonder why the warning is about .accnos file. Does cluster.split use a .accnos file?

somebody to help me, please?

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [March 21, 2023, 6:26pm UTC](https://forum.mothur.org/t/problem-cluster-split/21760/2 "2023-03-21T18:26:40Z")

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Is this the same dataset as you were struggling with on the other thread? Can you revisit this once you get that thread resolved?

Pat

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**Author:** ![PeterJS0126](https://avatars.discourse-cdn.com/v4/letter/p/b4bc9f/32.png) [@PeterJS0126](https://forum.mothur.org/u/PeterJS0126)\
**Post date:** [March 26, 2023, 3:36am UTC](https://forum.mothur.org/t/problem-cluster-split/21760/3 "2023-03-26T03:36:20Z")

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> [@pschloss](#):
>
> Is this the same dataset as you were struggling with on the other thread?

This is my first thread…what do you mean with "the other thread’'?

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [March 27, 2023, 5:50pm UTC](https://forum.mothur.org/t/problem-cluster-split/21760/4 "2023-03-27T17:50:06Z")

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Ack - sorry, I got you confused with someone else.

Can you post the command as you are running it along with which version number and operating system you are using?

Pat

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**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\
**Post date:** [April 6, 2023, 5:51pm UTC](https://forum.mothur.org/t/problem-cluster-split/21760/5 "2023-04-06T17:51:05Z")

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Closed on April 6, 2023, 5:51pm UTC

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