I was trying to run tax4fun from my mothur output file. From your post, I came to know that, you used Tax4fun for functional prediction. I tried it but got bugs. I used mothur and used silva132 version for otu cluster and classification. I couldn’t proceed further with tax4fun. Is there any way that I can email you so that I can share my bugs and issues?
I converted by shared file to biom file in mothur and then converted by biom file to txt using Qiime2
and used that txt file for tax4fun
file_collapsed<-Tax4Fun(file,folderReferenceData,fctProfiling = FALSE, refProfile = “UProC”,shortReadMode = TRUE,normCopyNo = TRUE)
Thank you so much. your help will be highly appreciated.