# Pairwise.seq with version 1.46.1

**URL:** <https://forum.mothur.org/t/pairwise-seq-with-version-1-46-1/21136>\
**Category:** Uncategorized\
**Created:** [September 17, 2021, 10:36am UTC](https://forum.mothur.org/t/pairwise-seq-with-version-1-46-1/21136 "2021-09-17T10:36:29Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Hzsun](https://avatars.discourse-cdn.com/v4/letter/h/a698b9/32.png) [@Hzsun](https://forum.mothur.org/u/Hzsun)\
**Post date:** [September 17, 2021, 10:36am UTC](https://forum.mothur.org/t/pairwise-seq-with-version-1-46-1/21136/1 "2021-09-17T10:36:29Z")

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Hei,  
I am using pairwise.seq in version 1.46.1 to re-run previous ITS sequence with cutoff=0.03, which suppose to take much short time as the kmercutoff parameter was added. However, the generated .dist file is too large when using multiple processor, the process is always killed. I have used version 1.45 to run pairwise.seq for the same dataset, it worked fine. Is it possible bug in the command in the new version not using multiple processors?

below is the batch job:  
#SBATCH -p longrun  
#SBATCH -J pairwise.seq  
#SBATCH -o output\_%j  
#SBATCH -e error\_%j  
#SBATCH -n 1  
#SBATCH --nodes=1  
#SBATCH -t 200:00:00  
#SBATCH --cpus-per-task=10  
#SBATCH --mem-per-cpu=30000  
#SBATCH --mail-type=END

pairwise.seqs(fasta=stability.trim.contigs.pcr.good.unique.pick.trim.precluster.fasta,cutoff=0.03,processors=10)

Thanks,

Hui

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [October 1, 2021, 5:28pm UTC](https://forum.mothur.org/t/pairwise-seq-with-version-1-46-1/21136/2 "2021-10-01T17:28:02Z")

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Could you send your input files and logfile to [mothur.bugs@gmail.com](mailto:mothur.bugs@gmail.com) so I can track down the issue for you?