# No groups file from trim.seqs

**URL:** https://forum.mothur.org/t/no-groups-file-from-trim-seqs/247
**Category:** Commands in mothur
**Created:** [June 17, 2010, 9:49pm UTC](https://forum.mothur.org/t/no-groups-file-from-trim-seqs/247 "2010-06-17T21:49:49Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![tyty](https://avatars.discourse-cdn.com/v4/letter/t/35a633/32.png) [@tyty](https://forum.mothur.org/u/tyty)
#### Post date: [June 17, 2010, 9:49pm UTC](https://forum.mothur.org/t/no-groups-file-from-trim-seqs/247/1 "2010-06-17T21:49:49Z")

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I’m not sure what I am doing wrong exactly, but I was never able to get the groups file you need to generate the otus after running the trim.seqs command. I was wondering if anyone has an idea about why this is happening.

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### Author: ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)
#### Post date: [June 18, 2010, 12:42pm UTC](https://forum.mothur.org/t/no-groups-file-from-trim-seqs/247/2 "2010-06-18T12:42:51Z")

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Can you write out how you are using the trim.seqs command? If you forget to include the oligos file you won’t get a groups file.

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### Author: ![tyty](https://avatars.discourse-cdn.com/v4/letter/t/35a633/32.png) [@tyty](https://forum.mothur.org/u/tyty)
#### Post date: [June 23, 2010, 10:38am UTC](https://forum.mothur.org/t/no-groups-file-from-trim-seqs/247/3 "2010-06-23T10:38:54Z")

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That was actually the problem. Thanks!
