# No generation of a stability.trim.contigs.good.count\_table

**URL:** https://forum.mothur.org/t/no-generation-of-a-stability-trim-contigs-good-count-table/21500
**Category:** Uncategorized
**Created:** [July 24, 2022, 9:58pm UTC](https://forum.mothur.org/t/no-generation-of-a-stability-trim-contigs-good-count-table/21500 "2022-07-24T21:58:09Z")
**Posts on this page:** 2
**Page:** 1

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### Author: ![nicoletraver](https://avatars.discourse-cdn.com/v4/letter/n/838e76/32.png) [@nicoletraver](https://forum.mothur.org/u/nicoletraver)
#### Post date: [July 24, 2022, 9:58pm UTC](https://forum.mothur.org/t/no-generation-of-a-stability-trim-contigs-good-count-table/21500/1 "2022-07-24T21:58:09Z")

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Hi Everyone,

I was wondering if anyone would be able to help me with a problem I am having. I am using the example data on mothur to work through the MiSeq SOP. Everything has ran smoothly until I have gotten to here:

mothur \> screen.seqs(fasta=stability.trim.contigs.unique.align, count=stability.trim.contigs.count\_table, start=1969, end=11551)

It gives me an error that a sequence isn’t in my count table, but when I look in the file that specific sequence is there. I also noticed that I am missing a stability.trim.contigs.good.count\_table file which I need for the following line and the sequential ones after it:

mothur \> unique.seqs(fasta=stability.trim.contigs.unique.good.filter.fasta, count=stability.trim.contigs.good.count\_table)

I’m not sure why this file can’t be generated. If anyone could help me, I would really appreciate it!

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### Author: ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)
#### Post date: [August 2, 2022, 3:38pm UTC](https://forum.mothur.org/t/no-generation-of-a-stability-trim-contigs-good-count-table/21500/2 "2022-08-02T15:38:06Z")

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Hi Nicole - is this with the MiSeq data or your own data? What usually works best is to go back a step or two until you no longer get the error messages and then rerun the following commands. Errors like your typically happen because a file gets left out of a command where sequences are removed. I wonder if you included a count file when you ran `unique.seqs`

Pat