# Mothur run aborted

**URL:** <https://forum.mothur.org/t/mothur-run-aborted/22190>\
**Category:** Uncategorized\
**Created:** [July 25, 2024, 5:57pm UTC](https://forum.mothur.org/t/mothur-run-aborted/22190 "2024-07-25T17:57:39Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![jrthelusmond](https://avatars.discourse-cdn.com/v4/letter/j/ecd19e/32.png) [@jrthelusmond](https://forum.mothur.org/u/jrthelusmond)\
**Post date:** [July 25, 2024, 5:57pm UTC](https://forum.mothur.org/t/mothur-run-aborted/22190/1 "2024-07-25T17:57:39Z")

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Hello everyone, I have been trying to use Mothur to process 16S rRNA sequence data. Whenever I got to the line of codes below, the run is aborted. Have you encountered this before? Your assistance is highly appreciated. Jean-Rene  
cluster.split(fasta=current, count=current, taxonomy=current, splitmethod=classify, taxlevel=4, cutoff=0.15)

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [July 30, 2024, 1:02pm UTC](https://forum.mothur.org/t/mothur-run-aborted/22190/2 "2024-07-30T13:02:18Z")

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Hi there,

I suspect you are using up all of the RAM on your computer. Can you tell me what region you are sequencing? You might try to drop the cutoff to 0.03, which should help a bit.

Pat