# make.sra for sff question

**URL:** <https://forum.mothur.org/t/make-sra-for-sff-question/2398>\
**Category:** Commands in mothur\
**Created:** [June 12, 2015, 6:45pm UTC](https://forum.mothur.org/t/make-sra-for-sff-question/2398 "2015-06-12T18:45:58Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![cbassis](https://avatars.discourse-cdn.com/v4/letter/c/a6a055/32.png) [@cbassis](https://forum.mothur.org/u/cbassis)\
**Post date:** [June 12, 2015, 6:45pm UTC](https://forum.mothur.org/t/make-sra-for-sff-question/2398/1 "2015-06-12T18:45:58Z")

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I used make.sra to prepare sff and xml files for SRA submission.  
I have 2 questions:

1. Do you recommend using the same pdiffs and bdiffs as for my analysis? On my first go I just left the defaults but maybe I should change this.
2. The xml output included the .scrap.sff files (these were not in my mimark files) -is there a way to avoid this? The SRA would like it out of the xml or they would like the .scrap.sff file which I don’t think I want to submit.  
Thanks!

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [June 16, 2015, 1:37pm UTC](https://forum.mothur.org/t/make-sra-for-sff-question/2398/2 "2015-06-16T13:37:12Z")

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> 1. Do you recommend using the same pdiffs and bdiffs as for my analysis? On my first go I just left the defaults but maybe I should change this.

Yeah, i would use the same pdiffs/bdiffs

> 1. The xml output included the .scrap.sff files (these were not in my mimark files) -is there a way to avoid this? The SRA would like it out of the xml or they would like the .scrap.sff file which I don’t think I want to submit.

Let me have Sarah get back to you on this one…

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [June 16, 2015, 6:30pm UTC](https://forum.mothur.org/t/make-sra-for-sff-question/2398/3 "2015-06-16T18:30:42Z")

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Thanks for the feature request! We will add making scrap sequence submission optional to our list. If you send me your xml file I can remove the scrap references for you, [mothur.bugs@gmail.com](mailto:mothur.bugs@gmail.com).
